Taste neurons consist of both a large TrkB-receptor-dependent and a small TrkB-receptor-independent subpopulation.
Taste neurons consist of both a large TrkB-receptor-dependent and a small TrkB-receptor-independent subpopulation.
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味道神经元由大的TRKB受体依赖性和小型TRKB受体独立的亚群组成。
DOI：
10.1371/journal.pone.0083460
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Krimm RF
中科院分区：
文献类型：
--
作者：
Fei D;Krimm RF
脑源性神经营养因子(BDNF)和神经营养因子-4(NT-4)是两种神经营养因子，它们在膝状体(味觉)神经元存活、靶神经支配和味蕾形成中发挥着不同的作用。这两种神经营养因子都激活原肌球蛋白相关的激酶B(TrkB)受体和泛神经营养因子受体p75。虽然这些神经营养因子的作用已经被很好地研究了，但BDNF和NT-4在体内通过TrkB调节味觉发育的程度仍然不清楚。在这项研究中，我们比较了trkB−/−和bdnf−/−/ntf4−/−小鼠的味觉发育，以确定这些缺陷是否相似。如果是这样，这将表明BDNF和NT-4的功能都可以通过TrkB信号来解释。我们发现trkB−/−和bdnf−/−/ntf4−/−小鼠在胚胎13.5时丢失了相似数量的膝状神经元，这表明bdnf和NT-4主要通过trkB来调节膝状体神经元的存活。令人惊讶的是，与bdnf−/−/ntf4−/−小鼠中残留的神经元相比，trkB−/−小鼠中保留的少数膝状神经元在支配舌头和味蕾方面更成功。TrkB−/−小鼠体内剩余的神经元支持相当数量的味蕾。此外，这些剩余的神经元不表达TrkB受体，这表明BDNF或NT-4必须通过额外的受体来影响舌神经支配和/或靶向。
Brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4) are two neurotrophins that play distinct roles in geniculate (taste) neuron survival, target innervation, and taste bud formation. These two neurotrophins both activate the tropomyosin-related kinase B (TrkB) receptor and the pan-neurotrophin receptor p75. Although the roles of these neurotrophins have been well studied, the degree to which BDNF and NT-4 act via TrkB to regulate taste development in vivo remains unclear. In this study, we compared taste development in TrkB−/− and Bdnf−/−/Ntf4−/− mice to determine if these deficits were similar. If so, this would indicate that the functions of both BDNF and NT-4 can be accounted for by TrkB-signaling. We found that TrkB−/− and Bdnf−/−/Ntf4−/− mice lose a similar number of geniculate neurons by E13.5, which indicates that both BDNF and NT-4 act primarily via TrkB to regulate geniculate neuron survival. Surprisingly, the few geniculate neurons that remain in TrkB−/− mice are more successful at innervating the tongue and taste buds compared with those neurons that remain in Bdnf−/−/Ntf4−/− mice. The remaining neurons in TrkB−/− mice support a significant number of taste buds. In addition, these remaining neurons do not express the TrkB receptor, which indicates that either BDNF or NT-4 must act via additional receptors to influence tongue innervation and/or targeting.
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影响因子：
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