Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200
Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200
复制标题
基因组和生理分析揭示深海发光杆菌 ANT-2200 的多功能代谢能力
DOI：
10.1007/s00792-016-0822-1
复制
发表时间：
2016-04
期刊：
影响因子：
2.9
通讯作者：
Alberto Francoi
中科院分区：
文献类型：
--
作者：
Zhang Sheng-Da;Santini Claire-Lise;Zhang Wei-Jia;Barbe Valerie;Mangenot Sophie;Guyomar Charlotte;Garel Marc;Chen Hai-Tao;Li Xue-Gong;Yin Qun-Jian;Zhao Yuan;Armengaud Jean;Gaillard Jean-Charles;Martini Severine;Pradel Nathalie;Vidaud Claude;Alberto Francoi
发光杆菌属细菌在世界各地的海洋中繁衍生息，并表现出巨大的生态生理多样性，包括自由生活、共生和嗜压生活方式。人们对这种跨物种变异性背后的基因组特征知之甚少。在这里，我们对发光杆菌ANT-2200菌株进行了基因组和生理分析，这是第一个基因组已测序的深海发光细菌。使用光学绘图，我们更新了基因组数据并将其重新组装成两条染色体和一个大质粒。基因组分析揭示了其多功能的能量代谢潜力，生理分析证实了其生长能力，通过从葡萄糖或麦芽糖发酵中获取能量，通过以甲酸盐作为电子供体和三甲胺N-氧化物（TMAO）、硝酸盐或富马酸盐作为电子受体的呼吸，或通过在丰富培养基中的化学有机异养生长。尽管 ANT-2200 菌株是在溶解氧饱和的位置分离的，但它拥有四个编码典型厌氧酶（TMAO 还原酶）的基因簇。静水压升高会增强TMAO还原酶活性，主要是由于同工酶TorA1的增加。 TMAO 还原酶同工酶的高拷贝数和压力增强的活性可能意味着细菌为适应深海栖息地而开发的策略，其中即时 TMAO 可用性可能随深度而增加。
Bacteria of the genus Photobacterium thrive worldwide in oceans and show substantial eco-physiological diversity including free-living, symbiotic and piezophilic life styles. Genomic characteristics underlying this variability across species are poorly understood. Here we carried out genomic and physiological analysis of Photobacterium phosphoreum strain ANT-2200, the first deep-sea luminous bacterium of which the genome has been sequenced. Using optical mapping we updated the genomic data and reassembled it into two chromosomes and a large plasmid. Genomic analysis revealed a versatile energy metabolic potential and physiological analysis confirmed its growth capacity by deriving energy from fermentation of glucose or maltose, by respiration with formate as electron donor and trimethlyamine N-oxide (TMAO), nitrate or fumarate as electron acceptors, or by chemo-organo-heterotrophic growth in rich media. Despite that it was isolated at a site with saturated dissolved oxygen, the ANT-2200 strain possesses four gene clusters coding for typical anaerobic enzymes, the TMAO reductases. Elevated hydrostatic pressure enhances the TMAO reductase activity, mainly due to the increase of isoenzyme TorA1. The high copy number of the TMAO reductase isoenzymes and pressure-enhanced activity might imply a strategy developed by bacteria to adapt to deep-sea habitats where the instant TMAO availability may increase with depth.
登录
查看更多内容
影响因子：
3.7
作者：
Martini S;Al Ali B;Garel M;Nerini D;Grossi V;Pacton M;Casalot L;Cuny P;Tamburini C
通讯作者：
Tamburini C
DOI：
10.1016/s1095-6433(02)00182-4
发表时间：
2002-11
期刊：
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
影响因子：
--
作者：
P. Yancey;W. R. Blake;J. Conley
通讯作者：
P. Yancey;W. R. Blake;J. Conley
影响因子：
3.2
作者：
Ansaldi, M;Jourlin-Castelli, C;Méjean, V
通讯作者：
Méjean, V
影响因子：
11.4
作者：
Santini, CL;Ize, B;Wu, LF
通讯作者：
Wu, LF
影响因子：
2.5
作者：
Al Ali, Badr;Garel, Marc;Tamburini, Christian
通讯作者：
Tamburini, Christian