Tuning supramolecular rigidity of peptide fibers through molecular structure.
Tuning supramolecular rigidity of peptide fibers through molecular structure.
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DOI：
10.1021/ja908560n
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发表时间：
2010-05-05
影响因子：
15
通讯作者：
Stupp, Samuel I.
中科院分区：
文献类型：
--
作者：
Pashuck, E. Thomas;Cui, Honggang;Stupp, Samuel I.
我们合成了一系列具有系统修饰氨基酸序列的肽两亲体（PAs），以控制它们通过自组装形成的纳米纤维凝胶的力学性能。通过控制肽序列中缬氨酸和丙氨酸的数量和位置，我们发现缬氨酸增加了凝胶的刚度，而额外的丙氨酸降低了凝胶的力学性能。玻璃冰晶透射电镜显示，本文研究的所有PA分子形成直径8 - 10nm，长度几微米的纳米纤维。我们通过傅里叶变换红外实验发现，凝胶刚度和沿着纤维长轴的氢键排列之间有很强的相关性。圆二色性发现，形成机械刚度最高的超分子结构的分子自组装成β-片，其扭曲和无序程度最小，这与红外实验结果一致。分子控制三维人工肽两亲基质的机械刚度为控制干细胞分化和细胞形态等生物现象提供了一种化学策略。
We synthesized a series of peptide amphiphiles (PAs) with systematically modified amino acid sequences to control the mechanical properties of the nanofiber gels they form by self-assembly. By manipulating the number and position of valines and alanines in the peptide sequence we found that valines increase the stiffness of the gel while additional alanines decrease the mechanical properties. Vitreous ice cryo-transmission electron microscopy shows that all PA molecules investigated here form nanofibers 8–10 nm in diameter and several micrometers in length. We found through Fourier transform IR experiments a strong correlation between gel stiffness and hydrogen bond alignment along the long axis of the fiber. Molecules that form supramolecular structures with the highest mechanical stiffness were found by circular dichroism to self-assemble into β-sheets with the least amount of twisting and disorder, a result which is consistent with IR experiments. Molecular control of mechanical stiffness in three-dimensional artificial peptide amphiphile matrices offers a chemical strategy to control biological phenomena such as stem cell differentiation and cell morphology.
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影响因子：
64.8
作者：
Aggeli, A;Bell, M;Radford, SE
通讯作者：
Radford, SE
影响因子：
--
作者：
Aggeli, A;Bell, M;Semenov, A
通讯作者：
Semenov, A
DOI：
10.1073/pnas.0701980104
发表时间：
2007-05-08
影响因子：
11.1
作者：
Haines-Butterick, Lisa;Rajagopal, Karthikan;Schneider, Joel P.
通讯作者：
Schneider, Joel P.
纳米神经编织：用于大脑修复和轴突再生以及视力功能恢复的肽纳米纤维支架
DOI：
10.1073/pnas.0600559103
发表时间：
2006-03-28
影响因子：
11.1
作者：
Ellis-Behnke, RG;Liang, YX;Schneider, GE
通讯作者：
Schneider, GE
DOI：
10.1083/jcb.200405004
发表时间：
2004-09-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Engler AJ;Griffin MA;Sen S;Bönnemann CG;Sweeney HL;Discher DE
通讯作者：
Discher DE