Evolution of light-harvesting complex proteins from Chl c-containing algae.
Evolution of light-harvesting complex proteins from Chl c-containing algae.
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DOI：
10.1186/1471-2148-11-101
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发表时间：
2011-04-15
影响因子：
3.4
通讯作者：
Delwiche CF
中科院分区：
文献类型：
--
作者：
Hoffman GE;Sanchez Puerta MV;Delwiche CF
光捕获复合体(LHC)蛋白通过结合吸收光的叶绿素(Chl)和类胡萝卜素分子并将能量传递到光系统的反应中心Chl而在光合作用中发挥作用。大多数研究集中在植物和叶绿素a和b结合的LHC上，对这些蛋白质的广泛研究揭示了各种生化功能、表达模式和氨基酸序列。我们在这里重点介绍一个研究较少的LHC家族，它们通常结合Chla和c，并且广泛分布于含Chl c的藻类和其他藻类中。以前对这些蛋白质的系统发育分析表明，单个藻类谱系拥有来自一个或两个亚科的蛋白质，大多数亚科具有特定藻类谱系的特征，但基因组规模的数据集显示，一些物种具有多种不同形式的基因。这些观察还表明，内共生可能对LHC的进化产生了重要影响。我们使用最近测序项目的数据重建了含Chl-c藻类和相关谱系的LHC的系统发育，提供了比以前研究大10倍的分类单元样本。系统发育分析表明，单个类群拥有来自多个LHC亚家族的蛋白质，在远缘关系较远的藻类谱系中发现了几个LHC亚家族。这种系统发育模式意味着基因家族的功能分化，这一假设与基因表达、类胡萝卜素结合和与其他LHC的物理联系的数据一致。在所有的可能性中，LHC经历了一段复杂的功能进化、基因转移和谱系特异性多样化的历史。这一分析提供了一幅与之前对大型强子对撞机演化的分析截然不同的大型强子对撞机多样性的图景。单个藻类拥有来自多个LHC亚家族的蛋白质。进化关系支持叶绿体起源的假说。这项工作还允许在更广泛的系统发育背景下理解最近关于分子功能的实验发现。
Light harvesting complex (LHC) proteins function in photosynthesis by binding chlorophyll (Chl) and carotenoid molecules that absorb light and transfer the energy to the reaction center Chl of the photosystem. Most research has focused on LHCs of plants and chlorophytes that bind Chl a and b and extensive work on these proteins has uncovered a diversity of biochemical functions, expression patterns and amino acid sequences. We focus here on a less-studied family of LHCs that typically bind Chl a and c, and that are widely distributed in Chl c-containing and other algae. Previous phylogenetic analyses of these proteins suggested that individual algal lineages possess proteins from one or two subfamilies, and that most subfamilies are characteristic of a particular algal lineage, but genome-scale datasets had revealed that some species have multiple different forms of the gene. Such observations also suggested that there might have been an important influence of endosymbiosis in the evolution of LHCs. We reconstruct a phylogeny of LHCs from Chl c-containing algae and related lineages using data from recent sequencing projects to give ~10-fold larger taxon sampling than previous studies. The phylogeny indicates that individual taxa possess proteins from multiple LHC subfamilies and that several LHC subfamilies are found in distantly related algal lineages. This phylogenetic pattern implies functional differentiation of the gene families, a hypothesis that is consistent with data on gene expression, carotenoid binding and physical associations with other LHCs. In all probability LHCs have undergone a complex history of evolution of function, gene transfer, and lineage-specific diversification. The analysis provides a strikingly different picture of LHC diversity than previous analyses of LHC evolution. Individual algal lineages possess proteins from multiple LHC subfamilies. Evolutionary relationships showed support for the hypothesized origin of Chl c plastids. This work also allows recent experimental findings about molecular function to be understood in a broader phylogenetic context.
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