Oxidized alginate hydrogels as niche environments for corneal epithelial cells.
Oxidized alginate hydrogels as niche environments for corneal epithelial cells.
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DOI：
10.1002/jbm.a.35011
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发表时间：
2014-10
影响因子：
4.9
通讯作者：
Connon, Che J.
中科院分区：
文献类型：
--
作者：
Wright, Bernice;De Bank, Paul A.;Luetchford, Kim A.;Acosta, Fernando R.;Connon, Che J.
关键词：
水凝胶的化学和生物化学修饰是产生维持细胞功能的生理结构的一种策略。本研究的目的是应用氧化的藻酸盐水凝胶作为开发角膜缘上皮干细胞的仿生生态位的基础，该仿生生态位可用于治疗角膜功能障碍。干细胞表型的牛角膜缘上皮细胞（LEC）和角膜上皮细胞（CEC）的活力进行了检查，在氧化藻酸盐凝胶含有胶原IV超过3天的培养期。氧化增加了细胞活力（P ≤ 0.05），加入IV型胶原后进一步改善（P ≤ 0.01）。氧化凝胶的内孔（直径：0.2-0.8 µm）比未改性凝胶（孔径：0.05-0.1 µm）更大，硬度显著降低（P ≤ 0.001），表明孔径增加和硬度降低有助于提高细胞活力。胶原IV从氧化的藻酸盐凝胶的扩散是类似的未修饰的凝胶，这表明氧化可能不会影响藻酸盐凝胶中的细胞外基质蛋白的保留。这些数据表明，含有角膜细胞外基质蛋白的氧化藻酸盐凝胶可以影响角膜上皮细胞功能，其方式可能有益于角膜伤口愈合治疗。© 2013作者。生物医学材料研究杂志A部分由威利期刊公司出版。Part A：102A：3393-3400，2014.
Chemical and biochemical modification of hydrogels is one strategy to create physiological constructs that maintain cell function. The aim of this study was to apply oxidised alginate hydrogels as a basis for development of a biomimetic niche for limbal epithelial stem cells that may be applied to treating corneal dysfunction. The stem phenotype of bovine limbal epithelial cells (LEC) and the viability of corneal epithelial cells (CEC) were examined in oxidised alginate gels containing collagen IV over a 3-day culture period. Oxidation increased cell viability (P ≤ 0.05) and this improved further with addition of collagen IV (P ≤ 0.01). Oxidised gels presented larger internal pores (diameter: 0.2–0.8 µm) than unmodified gels (pore diameter: 0.05–0.1 µm) and were significantly less stiff (P ≤ 0.001), indicating that an increase in pore size and a decrease in stiffness contributed to improved cell viability. The diffusion of collagen IV from oxidised alginate gels was similar to that of unmodified gels suggesting that oxidation may not affect the retention of extracellular matrix proteins in alginate gels. These data demonstrate that oxidised alginate gels containing corneal extracellular matrix proteins can influence corneal epithelial cell function in a manner that may impact beneficially on corneal wound healing therapy. © 2013 The Authors. Journal of Biomedical Materials Research Part A Published byWiley Periodicals, Inc. Part A: 102A: 3393–3400, 2014.
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