Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells.
Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells.
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缺氧的 ADSC 衍生的 EV 促进软骨干/祖细胞的增殖和软骨分化
DOI：
10.1080/21623945.2021.1945210
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发表时间：
2021-12
期刊：
影响因子：
3.3
通讯作者：
Liu K
中科院分区：
文献类型：
--
作者：
Xue K;Jiang Y;Zhang X;Wu J;Qi L;Liu K
摘要软骨组织工程是修复软骨缺损的一个很有前途的选择，尽管收获大量的种子细胞仍然是一个主要的挑战。软骨干/祖细胞（CSPC）似乎是一个有前途的细胞来源。细胞外小泡（EV）可能在细胞与细胞、组织与组织之间的通讯中起重要作用。在本研究中，我们的目的是评估缺氧脂肪干细胞（ADSCs）衍生的EV对CSPC增殖和分化的影响。鉴定ADSC衍生的EV的特征，并在存在或不存在缺氧ADSC衍生的EV的情况下测量CSPC的增殖、迁移和软骨相关基因表达。通过扫描电子显微镜、组织学染色、生化和生物力学分析来评估缺氧ADSC衍生的EV对藻酸盐水凝胶培养中CSPC的影响。结果表明，大多数ADSC衍生的EV表现出直径为40-1000 nm的圆形或杯形形态，并表达CD 9、CD 63和CD 81。低氧ADSC衍生的EV增强了CSPC的迁移和增殖，这也增加了软骨相关基因的表达。低氧ADSC衍生的EV诱导CSPC产生显著更多的软骨基质和蛋白多糖。总之，低氧ADSC衍生的EV促进了软骨组织工程中CSPC的增殖和成软骨分化。
ABSTRACT Cartilage tissue engineering is a promising option for repairing cartilage defects, although harvesting a large number of seeding cells remains a major challenge. Cartilage stem/progenitor cells (CSPCs) seem to be a promising cell source. Hypoxic extracellular vesicles (EVs) may play a major role in cell-cell and tissue-tissue communication. In the current study, we aimed to evaluate the effect of hypoxic adipose-derived stem cells (ADSCs)-derived EVs on CSPCs proliferation and differentiation. The characteristics of ADSCs-derived EVs were identified, and proliferation, migration, and cartilage-related gene expression of CSPCs were measured with or without the presence of hypoxic ADSCs-derived EVs. SEM, histological staining, biochemical and biomechanical analysis was performed to evaluate the effect of hypoxic ADSCs-derived EVs on CSPCs in alginate hydrogel culture. The results indicated that the majority of ADSC-derived EVs exhibited a round-shaped or cup-shaped morphology with a diameter of 40–1000 nm and expressed CD9, CD63, and CD81. CSPCs migration and proliferation were enhanced by hypoxic ADSCs-derived EVs, which also increased the expression of cartilage-related genes. The hypoxic ADSCs-derived EVs induce CSPCs to produce significantly more cartilage matrix and proteoglycan. In conclusion, hypoxic ADSCs-derived EVs improved the proliferation and chondrogenic differentiation of CSPCs for cartilage tissue engineering.
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DOI：
10.1073/pnas.1521230113
发表时间：
2016-02-23
影响因子：
11.1
作者：
Kowal, Joanna;Arras, Guillaume;Thery, Clotilde
通讯作者：
Thery, Clotilde
影响因子：
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Noël D
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4
作者：
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通讯作者：
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DOI：
10.1007/978-1-4939-6728-5_4
发表时间：
2017-01-01
期刊：
EXOSOMES AND MICROVESICLES: METHODS AND PROTOCOLS
影响因子：
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作者：
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通讯作者：
Brisson, Alain R.