Dorsal lip maturation and initial archenteron extension depend on Wnt11 family ligands.
Dorsal lip maturation and initial archenteron extension depend on Wnt11 family ligands.
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DOI：
10.1016/j.ydbio.2022.10.013
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发表时间：
2023-01
影响因子：
2.7
通讯作者：
Kirschner, Marc W.
中科院分区：
文献类型：
--
作者：
Itallie, Elizabeth S. Van;Field, Christine M.;Mitchison, Timothy J.;Kirschner, Marc W.
Wnt 11家族蛋白是激活一种Dishevelled介导的非经典Wnt信号通路的配体。在所有脊椎动物中，功能丧失导致原肠胚形成和/或前后轴延伸缺陷。非哺乳类脊椎动物基因组编码两个Wnt 11家族蛋白，其不同的功能尚不清楚。我们在非洲爪蟾中分别和一起敲除了Wnt 11b和Wnt 11。单morphant表现出非常相似的表型延迟胚孔关闭，但他们有不同的表型在尾芽期。为了回应它们非常相似的原肠胚形成表型，我们选择了双morphants的特点。使用暗场照明的延时成像和记录仪分析，我们确定了一个失败的背侧囊胚唇成熟，与较慢的囊胚关闭和失败的内在化内胚层在背侧囊胚唇。我们将这些外部可见的表型与内部组织中的细胞事件联系起来，通过对苯胺和微管染色的完整固定胚胎进行成像。我们发现原肠的初始延伸与囊胚唇成熟相关，并且原肠延伸被Wnt 11家族信号传导减少显著破坏。我们在我们的免疫荧光解释的帮助下，新的，近膜位置的卵裂沟蛋白苯胺在囊胚唇和早期原肠上皮细胞。
Wnt11 family proteins are ligands that activate a type of Dishevelled-mediated, non-canonical Wnt signaling pathway. Loss of function causes defects in gastrulation and/or anterior-posterior axis extension in all vertebrates. Non-mammalian vertebrate genomes encode two Wnt11 family proteins whose distinct functions have been unclear. We knocked down Wnt11b and Wnt11, separately and together, in Xenopus laevis. Single morphants exhibited very similar phenotypes of delayed blastopore closure, but they had different phenotypes during the tailbud period. In response to their very similar gastrulation phenotypes, we chose to characterize dual morphants. Using dark field illuminated time-lapse imaging and kymograph analysis, we identified a failure of dorsal blastopore lip maturation that correlated with slower blastopore closure and failure to internalize the endoderm at the dorsal blastopore lip. We connected these externally visible phenotypes to cellular events in the internal tissues by imaging intact fixed embryos stained for anillin and microtubules. We found that the initial extension of the archenteron is correlated with blastopore lip maturation, and archenteron extension is dramatically disrupted by decreased Wnt11 family signaling. We were aided in our interpretation of the immunofluorescence by the novel, membrane proximal location of the cleavage furrow protein anillin in the epithelium of the blastopore lip and early archenteron.
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影响因子：
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作者：
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