The CYP74 Gene Family in Watermelon: Genome-Wide Identification and Expression Profiling Under Hormonal Stress and Root-Knot Nematode Infection
The CYP74 Gene Family in Watermelon: Genome-Wide Identification and Expression Profiling Under Hormonal Stress and Root-Knot Nematode Infection
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西瓜CYP74基因家族：激素胁迫和根结线虫感染下的全基因组鉴定和表达谱
DOI：
10.3390/agronomy9120872
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发表时间：
2019-12
期刊：
影响因子：
--
通讯作者：
Yang Youxin
中科院分区：
文献类型：
--
作者：
Zhou Yong;Guang Yelan;Li Jingwen;Wang Fei;Golam Jalal Ahammed;Yang Youxin
丙二烯氧化物合酶（AOS）和氢过氧化物裂解酶（HPL）是CYP 74基因家族的成员，是氧脂素途径的分支，在植物对多种逆境胁迫的应答中起着重要作用。本研究在西瓜基因组中发现了4个HPL基因和1个AOS基因，它们被聚为3个亚家族（CYP 74 A、CYP 74 B和CYP 74 C）。序列分析表明，大多数植物HPL和AOS蛋白都含有代表性的结构域，包括Helix-I区、Helix-K区（ExxR）和血红素结合结构域。在ClAOS和ClHPL基因的启动子区发现了许多与发育、胁迫和衰老相关的顺式元件，并且所检测到的ClAOS和ClHPL基因在不同组织和果实发育阶段以及对各种激素的响应中差异表达。此外，红光还能增强ClAOS在西瓜根结线虫病叶片和根部的表达，表明ClAOS在红光诱导的西瓜抗根结线虫病中起主要作用。这些结果为进一步了解西瓜CYP 74基因的功能奠定了基础。
Allene oxide synthase (AOS) and hydroperoxide lyase (HPL), members of the CYP74 gene family, are branches of the oxylipin pathway and play vital roles in plant responses to a number of stresses. In this study, four HPL genes and one AOS gene were identified in the watermelon genome, which were clustered into three subfamilies (CYP74A, CYP74B and CYP74C). Sequence analysis revealed that most HPL and AOS proteins from various plants contain representative domains, including Helix-I region, Helix-K region (ExxR) and Heme-binding domain. A number of development-, stress-, and hormone-related cis-elements were found in the promoter regions of the ClAOS and ClHPL genes, and the detected ClAOS and ClHPL genes were differentially expressed in different tissues and fruit development stages, as well as in response to various hormones. In addition, red light could enhance the expression of ClAOS in root-knot nematode-infected leaves and roots of watermelon, implying that ClAOS might play a primary role in red light-induced resistance against root-knot nematodes. These findings lay a foundation for understanding the specific function of CYP74 genes in watermelon.
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影响因子：
4.4
作者：
Yu J;Tehrim S;Wang L;Dossa K;Zhang X;Ke T;Liao B
通讯作者：
Liao B
影响因子：
5.6
作者：
Kyndt T;Nahar K;Haeck A;Verbeek R;Demeestere K;Gheysen G
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影响因子：
7.4
作者：
Howe, GA;Lee, GI;DeRocher, AE
通讯作者：
DeRocher, AE
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen
影响因子：
4.8
作者：
Glauser, Gaetan;Grata, Elia;Wolfender, Jean-Luc
通讯作者：
Wolfender, Jean-Luc