Global transcriptome profiles of Camellia sinensis during cold acclimation.
Global transcriptome profiles of Camellia sinensis during cold acclimation.
复制标题
茶树冷驯化过程中的整体转录组谱
DOI：
10.1186/1471-2164-14-415
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发表时间：
2013-06-22
期刊：
影响因子：
4.4
通讯作者：
Yang YJ
中科院分区：
文献类型：
--
作者：
Wang XC;Zhao QY;Ma CL;Zhang ZH;Cao HL;Kong YM;Yue C;Hao XY;Chen L;Ma JQ;Jin JQ;Li X;Yang YJ
背景
茶是世界上最受欢迎的非酒精健康饮料。茶树(Camellia sinensis(L.)需要进行冷驯化过程，以提高其冬季的耐寒性。茶树对低温的反应在分子水平上发生的变化还知之甚少。为了阐明茶树冷驯化的分子机制，我们利用RNA-Seq和数字基因表达(DGE)技术研究了茶树冷驯化过程中的全基因组表达谱。
结果
使用Illumina测序平台，我们获得了大约5735万个RNA-Seq读数。这些阅读片段被组装成216,831个转录本，平均长度为356个碱基对，N50为529个碱基。总共鉴定了1,770个差异表达的转录本，其中1,168个上调，602个下调。这些基因包括一组冷感受器或信号转导基因、冷反应转录因子基因、质膜稳定相关基因、渗透感应反应基因和解毒酶基因。DGE和定量RT-PCR分析进一步证实了RNA-Seq分析的结果。通径分析表明，“碳水化合物代谢途径”和“钙信号途径”可能在茶树对低温胁迫的响应中起重要作用。
结论
我们的研究对茶树在低温和非冰冻温度下的转录组图谱进行了全球调查，并对茶树在低温驯化过程中的分子机制进行了深入的研究。它还可以作为耐冷性相关研究的宝贵资源，并有助于探索与低温有关的基因，以提高对低温耐性和植物-环境相互作用的理解。
Background
Tea is the most popular non-alcoholic health beverage in the world. The tea plant (Camellia sinensis (L.) O. Kuntze) needs to undergo a cold acclimation process to enhance its freezing tolerance in winter. Changes that occur at the molecular level in response to low temperatures are poorly understood in tea plants. To elucidate the molecular mechanisms of cold acclimation, we employed RNA-Seq and digital gene expression (DGE) technologies to the study of genome-wide expression profiles during cold acclimation in tea plants.
Results
Using the Illumina sequencing platform, we obtained approximately 57.35 million RNA-Seq reads. These reads were assembled into 216,831 transcripts, with an average length of 356 bp and an N50 of 529 bp. In total, 1,770 differentially expressed transcripts were identified, of which 1,168 were up-regulated and 602 down-regulated. These include a group of cold sensor or signal transduction genes, cold-responsive transcription factor genes, plasma membrane stabilization related genes, osmosensing-responsive genes, and detoxification enzyme genes. DGE and quantitative RT-PCR analysis further confirmed the results from RNA-Seq analysis. Pathway analysis indicated that the “carbohydrate metabolism pathway” and the “calcium signaling pathway” might play a vital role in tea plants’ responses to cold stress.
Conclusions
Our study presents a global survey of transcriptome profiles of tea plants in response to low, non-freezing temperatures and yields insights into the molecular mechanisms of tea plants during the cold acclimation process. It could also serve as a valuable resource for relevant research on cold-tolerance and help to explore the cold-related genes in improving the understanding of low-temperature tolerance and plant-environment interactions.
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影响因子：
7.4
作者：
GRIFFITH, M;BROWN, GN;HUNER, NPA
通讯作者：
HUNER, NPA
影响因子：
7.2
作者：
Gao, MJ;Schäfer, UA;Hannoufa, A
通讯作者：
Hannoufa, A
影响因子：
5.8
作者：
Conesa, A;Götz, S;Robles, M
通讯作者：
Robles, M
影响因子：
5.2
作者：
Jurczyk, Barbara;Rapacz, Marcin;Sasal, Monika
通讯作者：
Sasal, Monika
DOI：
10.1073/pnas.1107161108
发表时间：
2011-09-27
影响因子：
11.1
作者：
Catala, Rafael;Medina, Joaquin;Salinas, Julio
通讯作者：
Salinas, Julio