Cloning and characterization of an environmental DNA-derived gene cluster that encodes the biosynthesis of the antitumor substance BE-54017.
Cloning and characterization of an environmental DNA-derived gene cluster that encodes the biosynthesis of the antitumor substance BE-54017.
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DOI：
10.1021/ja2022653
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发表时间：
2011-07-06
影响因子：
15
通讯作者：
Brady, Sean F.
中科院分区：
文献类型：
--
作者：
Chang, Fang-Yuan;Brady, Sean F.
据预测，每克土壤中含有数千种独特的细菌。土壤DNA文库是生物合成多样性的大库，可以从中恢复和研究各种次生代谢物基因簇。利用设计用于氧色氨酸二聚化基因的引物筛选存档的土壤DNA文库，使我们能够鉴定和功能表征第一个吲哚色氨酸生物合成基因簇。本文报道了环境DNA衍生基因簇编码抗肿瘤物质BE-54017的生物合成的恢复和异源表达。转座子诱变鉴定出两种单加氧酶，AbeX1和AbeX2，负责将吲哚咔唑前体转化为BE-54017的吲哚色氨酸核心。
Soil is predicted to contain thousands of unique bacterial species per gram. Soil DNA libraries represent large reservoirs of biosynthetic diversity from which diverse secondary metabolite gene clusters can be recovered and studied. The screening of an archived soil DNA library using primers designed to target oxy-tryptophan dimerization genes allowed us to identify and functionally characterize the first indolotryptoline biosynthetic gene cluster. The recovery and heterologous expression of an environmental DNA derived gene cluster encoding the biosynthesis of the antitumor substance BE-54017 is reported here. Transposon mutagenesis identified two monooxygenases, AbeX1 and AbeX2, as being responsible for the transformation of an indolocarbazole precursor into the indolotryptoline core of BE-54017.
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DOI：
10.1016/j.bbagen.2009.11.014
发表时间：
2010-03-01
影响因子：
3
作者：
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通讯作者：
Lindberg, Diana
影响因子：
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作者：
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影响因子：
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通讯作者：
Thorson, Jon S.
影响因子：
3.3
作者：
Onaka, H;Taniguchi, S;Furumai, T
通讯作者：
Furumai, T
影响因子：
15
作者：
Feng, Zhiyang;Kim, Jeff H.;Brady, Sean F.
通讯作者：
Brady, Sean F.