Structured coculture of mesenchymal stem cells and disc cells enhances differentiation and proliferation.
Structured coculture of mesenchymal stem cells and disc cells enhances differentiation and proliferation.
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DOI：
10.1159/000332985
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Lotz JC
中科院分区：
文献类型：
--
作者：
Allon AA;Butcher K;Schneider RA;Lotz JC
在体内干细胞分化过程中，成熟细胞经常诱导附近干细胞的分化。因此，先前的研究表明，随机混合共培养可以帮助间充质干细胞（MSC）转化为髓核细胞（NPC）。然而，由于体内信号通常发生在相邻细胞层之间的异位，我们假设MSC和NPC之间结构上有组织的共培养将导致比单细胞类型对照和随机组织共培养更大的细胞分化和增殖。我们开发了一种新的双层细胞颗粒（BCP）系统，通过连续离心将MSC球体封闭在NPC外壳中。对照采用单细胞型微球和随机组织共培养微球。评估微球的DNA含量、基因表达和组织学。双层三维组织增强细胞增殖和分化。BCP的细胞增殖明显高于单一细胞类型和随机组织的颗粒。定量RT-PCR、组织学和原位杂交证实了BCP颗粒中MSC的分化程度高于单细胞型颗粒。与单细胞型或随机混合共培养对照相比，BCP培养体系可增加MSC的增殖和分化。
During in vivo stem cell differentiation, mature cells often induce the differentiation of nearby stem cells. Accordingly, prior studies indicate that a randomly mixed coculture can help transform mesenchymal stem cells (MSC) into Nucleus Pulposus cells (NPC). However, because in vivo signaling typically occurs heterotopically between adjacent cell layers, we hypothesized that a structurally organized coculture between MSC and NPC will result in greater cell differentiation and proliferation over single cell type controls and cocultures with random organization. We developed a novel bilaminar cell pellet (BCP) system where a sphere of MSC is enclosed in a shell of NPC by successive centrifugation. Controls were made using single-cell type pellets and coculture pellets with random organization. The pellets were evaluated for DNA content, gene expression, and histology. A bilaminar 3D organization enhanced cell proliferation and differentiation. BCP showed significantly more cell proliferation than pellets with one cell type and those with random organization. Enhanced differentiation of MSC within the BCP pellet relative to single cell type pellets was demonstrated by quantitative RT-PCR, histology, and in situ hybridization. The BCP culture system increases MSC proliferation and differentiation as compared to single-cell type or randomly mixed co-culture controls.
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DOI：
10.1242/dev.023309
发表时间：
2008-12
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Eames BF;Schneider RA
通讯作者：
Schneider RA
影响因子：
4
作者：
DAndrea, P;Vittur, F
通讯作者：
Vittur, F
影响因子：
4.1
作者：
Wei, Aiqun;Chung, Sylvia A.;Diwan, Ashish D.
通讯作者：
Diwan, Ashish D.
影响因子：
3
作者：
Sakai, D;Mochida, J;Hotta, T
通讯作者：
Hotta, T
影响因子：
5.5
作者：
Valiunas, V;Doronin, S;Cohen, IS
通讯作者：
Cohen, IS