Transcription of Satellite III non-coding RNAs is a general stress response in human cells.
Transcription of Satellite III non-coding RNAs is a general stress response in human cells.
复制标题
DOI：
10.1093/nar/gkm1056
复制
发表时间：
2008-02
影响因子：
14.9
通讯作者：
Biamonti G
中科院分区：
文献类型：
--
作者：
Valgardsdottir R;Chiodi I;Giordano M;Rossi A;Bazzini S;Ghigna C;Riva S;Biamonti G
In heat-shocked human cells, heat shock factor 1 activates transcription of tandem arrays of repetitive Satellite III (SatIII) DNA in pericentromeric heterochromatin. Satellite III RNAs remain associated with sites of transcription in nuclear stress bodies (nSBs). Here we use real-time RT-PCR to study the expression of these genomic regions. Transcription is highly asymmetrical and most of the transcripts contain the G-rich strand of the repeat. A low level of G-rich RNAs is detectable in unstressed cells and a 104-fold induction occurs after heat shock. G-rich RNAs are induced by a wide range of stress treatments including heavy metals, UV-C, oxidative and hyper-osmotic stress. Differences exist among stressing agents both for the kinetics and the extent of induction (>100- to 80.000-fold). In all cases, G-rich transcripts are associated with nSBs. On the contrary, C-rich transcripts are almost undetectable in unstressed cells and modestly increase after stress. Production of SatIII RNAs after hyper-osmotic stress depends on the Tonicity Element Binding Protein indicating that activation of the arrays is triggered by different transcription factors. This is the first example of a non-coding RNA whose transcription is controlled by different transcription factors under different growth conditions.
登录
查看更多内容
DOI：
10.1083/jcb.200109018
发表时间：
2002-03-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Jolly C;Konecny L;Grady DL;Kutskova YA;Cotto JJ;Morimoto RI;Vourc'h C
通讯作者：
Vourc'h C
影响因子：
64.5
作者：
Gilbert, N;Boyle, S;Bickmore, WA
通讯作者：
Bickmore, WA
应力诱导的卫星III重复转录。
影响因子：
7.8
作者：
Jolly, Caroline;Metz, Alexandra;Govin, Jerome;Vigneron, Marc;Turner, Bryan M;Khochbin, Saadi;Vourc'h, Claire
通讯作者：
Vourc'h, Claire
影响因子：
3.3
作者：
Denegri, M;Chiodi, I;Biamonti, G
通讯作者：
Biamonti, G
影响因子：
12.8
作者：
Heo, Jee-In;Lee, Mi-Suk;Kim, Jong-Il
通讯作者：
Kim, Jong-Il