Stanniocalcin-2 inhibits skeletal muscle growth and is upregulated in functional overload-induced hypertrophy.
Stanniocalcin-2 inhibits skeletal muscle growth and is upregulated in functional overload-induced hypertrophy.
复制标题
Stanniocalcin-2 抑制骨骼肌生长，并在功能超负荷引起的肥大中上调。
DOI：
10.14814/phy2.15793
复制
发表时间：
2023-08
影响因子：
2.5
通讯作者：
中科院分区：
文献类型：
--
作者：
斯钙素-2（Stanniocalcin-2，STC 2）最近通过遗传分析与人类肌肉质量变异性有关。在生物化学上，STC 2抑制金属蛋白酶PAPP-A的蛋白水解活性，PAPP-A通过上调胰岛素样生长因子（IGF）轴促进肌肉生长。目的是检查STC 2是否影响骨骼肌质量，并评估IGF轴如何介导由功能过载引起的肌肉肥大。我们比较了Stc 2 −/−（n = 21）和野生型（n = 15）小鼠之间的肌肉质量和肌纤维形态。然后，我们定量了接受4周单侧超负荷的野生型小鼠（n = 14）的跖肌中的IGF 1、IGF 2、IGF结合蛋白−4和−5（IGFBP-4、IGFBP-5）、PAPP-A和STC 2。 与野生型小鼠相比，Stc 2 −/−小鼠的肌肉质量增加了10%。这种增加是由更大的肌纤维横截面积介导的。过载增加了跖肌质量和IGF轴的组成部分，包括IGF 1（2.41倍，p = 0.0117），IGF 2（1.70倍，p = 0.0461），IGFBP-4（1.48倍，p = 0.0268），PAPP-A（1.30倍，p = 0.0154）和STC 2（1.28倍，p = 0.019）的数量。在这里，我们提供的证据表明，STC 2是肌肉生长的抑制剂上调，沿着其他成分的IGF轴，在过载诱导的肌肉肥大。
Stanniocalcin‐2 (STC2) has recently been implicated in human muscle mass variability by genetic analysis. Biochemically, STC2 inhibits the proteolytic activity of the metalloproteinase PAPP‐A, which promotes muscle growth by upregulating the insulin‐like growth factor (IGF) axis. The aim was to examine if STC2 affects skeletal muscle mass and to assess how the IGF axis mediates muscle hypertrophy induced by functional overload. We compared muscle mass and muscle fiber morphology between Stc2 −/− (n = 21) and wild‐type (n = 15) mice. We then quantified IGF1, IGF2, IGF binding proteins −4 and −5 (IGFBP‐4, IGFBP‐5), PAPP‐A and STC2 in plantaris muscles of wild‐type mice subjected to 4‐week unilateral overload (n = 14). Stc2 −/− mice showed up to 10% larger muscle mass compared with wild‐type mice. This increase was mediated by greater cross‐sectional area of muscle fibers. Overload increased plantaris mass and components of the IGF axis, including quantities of IGF1 (by 2.41‐fold, p = 0.0117), IGF2 (1.70‐fold, p = 0.0461), IGFBP‐4 (1.48‐fold, p = 0.0268), PAPP‐A (1.30‐fold, p = 0.0154) and STC2 (1.28‐fold, p = 0.019). Here we provide evidence that STC2 is an inhibitor of muscle growth upregulated, along with other components of the IGF axis, during overload‐induced muscle hypertrophy.
登录
查看更多内容
影响因子：
3.9
作者：
Harstad, Sara L.;Conover, Cheryl A.
通讯作者：
Conover, Cheryl A.
影响因子：
3.3
作者：
Adams, GR;Haddad, F;Baldwin, KM
通讯作者：
Baldwin, KM
影响因子：
3.3
作者：
Adams, GR;McCue, SA
通讯作者：
McCue, SA
影响因子：
3.5
作者：
Awede, B;Thissen, JP;Lebacq, J
通讯作者：
Lebacq, J
影响因子：
2.5
作者：
B端nger, L;Laidlaw, A;Hill, WG
通讯作者：
Hill, WG