An integrated genetic map based on four mapping populations and quantitative trait loci associated with economically important traits in watermelon (Citrullus lanatus).
An integrated genetic map based on four mapping populations and quantitative trait loci associated with economically important traits in watermelon (Citrullus lanatus).
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基于四个作图群体和与西瓜（Citrullus lanatus）重要经济性状相关的数量性状位点的综合遗传图谱
DOI：
10.1186/1471-2229-14-33
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发表时间：
2014-01-20
影响因子：
5.3
通讯作者：
Xu Y
中科院分区：
文献类型：
--
作者：
Ren Y;McGregor C;Zhang Y;Gong G;Zhang H;Guo S;Sun H;Cai W;Zhang J;Xu Y
背景
现代西瓜由于多年来对所需园艺品质的选择，栽培品种共有狭窄的遗传基础。C.羊毛是西瓜育种新等位基因的重要潜在来源，但成功将性状渗入优良品种的成功有限。由于缺乏高质量的遗传图谱，分子标记辅助选择（MAS）技术在西瓜育种中的应用还有待于实现。最近，一些有用的地图已经成为可用的，但这些地图有几个共同的标记，并使用不同的标记集，从而构建，使地图之间的整合和比较分析困难。本研究旨在利用单核苷酸多态性（SNP）锚标记构建中国对虾的遗传图谱。毛状的
结果
在高密度遗传图谱的框架下，通过合并来自四个独立的作图实验的数据，使用遗传多样性阵列的亲本系，其中包括西瓜的三个亚种，构建了一个集成的遗传图谱。利用4个图谱中的698个SSR标记、219个插入缺失标记、36个结构变异标记和386个SNP标记构建了一个整合图谱。该图谱包含1339个标记，总长度为798 cM，平均标记间距为0.6 cM。58个先前报道的数量性状位点（QTL）的12个性状在这些群体中也被整合到地图中。此外，还增加了糖锤度、果糖、葡萄糖和蔗糖的QTL。一些QTL与经济上重要的性状检测在不同的遗传背景映射到类似的基因组区域的综合图谱，这表明，这样的QTL是负责观察到的表型变异在广泛的西瓜种质。
结论
本文所述的整合图谱相对于先前的西瓜遗传图谱增强了基因组工具的效用。整合图谱中的大部分标记是SSR、InDel和SNP，它们很容易在实验室之间转移。此外，用于构建的集成图包括所有三个西瓜亚种的群体，使该集成图有用的育种性状的选择，QTL的鉴定，MAS，种质资源分析和商业杂交种子检测。
Background
Modern watermelon (Citrullus lanatus L.) cultivars share a narrow genetic base due to many years of selection for desirable horticultural qualities. Wild subspecies within C. lanatus are important potential sources of novel alleles for watermelon breeding, but successful trait introgression into elite cultivars has had limited success. The application of marker assisted selection (MAS) in watermelon is yet to be realized, mainly due to the past lack of high quality genetic maps. Recently, a number of useful maps have become available, however these maps have few common markers, and were constructed using different marker sets, thus, making integration and comparative analysis among maps difficult. The objective of this research was to use single-nucleotide polymorphism (SNP) anchor markers to construct an integrated genetic map for C. lanatus.
Results
Under the framework of the high density genetic map, an integrated genetic map was constructed by merging data from four independent mapping experiments using a genetically diverse array of parental lines, which included three subspecies of watermelon. The 698 simple sequence repeat (SSR), 219 insertion-deletion (InDel), 36 structure variation (SV) and 386 SNP markers from the four maps were used to construct an integrated map. This integrated map contained 1339 markers, spanning 798 cM with an average marker interval of 0.6 cM. Fifty-eight previously reported quantitative trait loci (QTL) for 12 traits in these populations were also integrated into the map. In addition, new QTL identified for brix, fructose, glucose and sucrose were added. Some QTL associated with economically important traits detected in different genetic backgrounds mapped to similar genomic regions of the integrated map, suggesting that such QTL are responsible for the phenotypic variability observed in a broad array of watermelon germplasm.
Conclusions
The integrated map described herein enhances the utility of genomic tools over previous watermelon genetic maps. A large proportion of the markers in the integrated map are SSRs, InDels and SNPs, which are easily transferable across laboratories. Moreover, the populations used to construct the integrated map include all three watermelon subspecies, making this integrated map useful for the selection of breeding traits, identification of QTL, MAS, analysis of germplasm and commercial hybrid seed detection.
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影响因子：
3.7
作者：
Ren Y;Zhang Z;Liu J;Staub JE;Han Y;Cheng Z;Li X;Lu J;Miao H;Kang H;Xie B;Gu X;Wang X;Du Y;Jin W;Huang S
通讯作者：
Huang S
影响因子：
3.3
作者：
Falque, M;Décousset, L;Murigneux, A
通讯作者：
Murigneux, A
DOI：
10.1017/s1479262108014366
发表时间：
2009-04-01
期刊：
Plant Genetic Resources Characterization and Utilization
影响因子：
--
作者：
Levi, Amnon;Wechter, Patrick;Davis, Angela
通讯作者：
Davis, Angela
影响因子：
5.4
作者：
Somers, DJ;Isaac, P;Edwards, K
通讯作者：
Edwards, K
整合了多个成分图谱和高通量多样性阵列技术 (DArT) 标记的高粱共识遗传图谱。
影响因子：
5.3
作者：
Mace ES;Rami JF;Bouchet S;Klein PE;Klein RR;Kilian A;Wenzl P;Xia L;Halloran K;Jordan DR
通讯作者：
Jordan DR