An integrated genetic and cytogenetic map of the cucumber genome.
An integrated genetic and cytogenetic map of the cucumber genome.
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DOI：
10.1371/journal.pone.0005795
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发表时间：
2009-06-04
期刊：
影响因子：
3.7
通讯作者：
Huang S
中科院分区：
文献类型：
--
作者：
Ren Y;Zhang Z;Liu J;Staub JE;Han Y;Cheng Z;Li X;Lu J;Miao H;Kang H;Xie B;Gu X;Wang X;Du Y;Jin W;Huang S
葫芦科包括黄瓜、甜瓜、西瓜、南瓜和南瓜等重要作物。然而，很少有遗传和基因组资源可用于植物改良。一些瓜类物种如黄瓜具有狭窄的遗传基础，这阻碍了饱和分子连锁图谱的构建。我们在此报告的发展高度多态性的简单重复序列（SSR）标记起源于全基因组鸟枪测序和随后的高密度遗传连锁图谱的构建。该图谱包括7个连锁群的995个SSR标记，总长度为573cM，确定了1680个重组断点，两标记间的平均重组断点为0.58cM。这些连锁群，然后分配到七个相应的染色体使用荧光原位杂交（FISH）。FISH分析也揭示了黄瓜属亚种间的染色体倒位[C.大蒜变种sativus L.和var。hardwickii（R.）Alef]，这导致遗传图谱上的标记聚类。在11个黄瓜自交系中，四分之一的定位标记显示出相对较高的多态性水平。在995个标记中，甜瓜、西瓜和南瓜的保守性分别为49%、26%和22%。该图谱的建立将为黄瓜全基因组测序、定位克隆和分子育种提供便利，并为瓜类基因和性状知识的整合奠定基础。
The Cucurbitaceae includes important crops such as cucumber, melon, watermelon, squash and pumpkin. However, few genetic and genomic resources are available for plant improvement. Some cucurbit species such as cucumber have a narrow genetic base, which impedes construction of saturated molecular linkage maps. We report herein the development of highly polymorphic simple sequence repeat (SSR) markers originated from whole genome shotgun sequencing and the subsequent construction of a high-density genetic linkage map. This map includes 995 SSRs in seven linkage groups which spans in total 573 cM, and defines ∼680 recombination breakpoints with an average of 0.58 cM between two markers. These linkage groups were then assigned to seven corresponding chromosomes using fluorescent in situ hybridization (FISH). FISH assays also revealed a chromosomal inversion between Cucumis subspecies [C. sativus var. sativus L. and var. hardwickii (R.) Alef], which resulted in marker clustering on the genetic map. A quarter of the mapped markers showed relatively high polymorphism levels among 11 inbred lines of cucumber. Among the 995 markers, 49%, 26% and 22% were conserved in melon, watermelon and pumpkin, respectively. This map will facilitate whole genome sequencing, positional cloning, and molecular breeding in cucumber, and enable the integration of knowledge of gene and trait in cucurbits.
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DOI：
10.1111/j.1471-8286.2006.01500.x
发表时间：
2006-12-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Kong, Q.;Xiang, C.;Yu, Z.
通讯作者：
Yu, Z.
影响因子：
2
作者：
Sharma, BD;Hore, DK
通讯作者：
Hore, DK
影响因子：
1.7
作者：
Han, Y. H.;Zhang, Z. H.;Jin, W. W.
通讯作者：
Jin, W. W.
影响因子：
5.4
作者：
KEIM, P;BEAVIS, W;FREESTONE, R
通讯作者：
FREESTONE, R
影响因子：
3.1
作者：
Danin-Poleg, Y;Reis, N;Katzir, N
通讯作者：
Katzir, N