Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain.
Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain.
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DOI：
10.1371/journal.pone.0001631
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发表时间：
2008-02-20
期刊：
影响因子：
3.7
通讯作者：
Landry CF
中科院分区：
文献类型：
--
作者：
Gerstner JR;Bremer QZ;Vander Heyden WM;Lavaute TM;Yin JC;Landry CF
脑脂肪酸结合蛋白（Fabp 7）在成熟脑中的星形胶质细胞和神经元细胞前体中表达，在早期神经系统发育中起重要作用。我们在这里报告，Fabp 7 mRNA在成年小鼠大脑中的水平在24小时内发生变化。与Fabp 5不同，Fabp 5是一种广泛表达于脑内各种细胞类型中的脂肪酸结合蛋白，RNA分析显示，Fabp 7 mRNA水平在涉及睡眠和活动机制的脑区域中在光照期间升高，在黑暗期间降低。这种模式的Fabp 7 mRNA的表达被证实使用原位杂交，并发现发生在整个大脑。Fabp 7 mRNA的细胞内分布的变化在24小时内也是明显的。然而，在出生后第6天的大脑中没有发现Fabp 7的昼夜变化，此时星形胶质细胞尚未成熟。相反，成年海马颗粒下区的颗粒细胞前体细胞Fabp 7表达发生昼夜变化。这些变化抑制了整个大脑中Fabp 7 mRNA的振荡，表明细胞协调信号可能控制全脑Fabp 7 mRNA的表达。免疫印迹显示，Fabp 7蛋白水平也经历了丰度的昼夜变化，峰值水平发生在黑暗时期。时钟或时钟调节的基因，Fabp 7表达的同步，全球循环模式是独特的，并牵连神经胶质细胞的活动和/或昼夜节律的响应或调制。
Brain fatty acid binding protein (Fabp7), which is important in early nervous system development, is expressed in astrocytes and neuronal cell precursors in mature brain. We report here that levels of Fabp7 mRNA in adult murine brain change over a 24 hour period. Unlike Fabp5, a fatty acid binding protein that is expressed widely in various cell types within brain, RNA analysis revealed that Fabp7 mRNA levels were elevated during the light period and lower during dark in brain regions involved in sleep and activity mechanisms. This pattern of Fabp7 mRNA expression was confirmed using in situ hybridization and found to occur throughout the entire brain. Changes in the intracellular distribution of Fabp7 mRNA were also evident over a 24 hour period. Diurnal changes in Fabp7, however, were not found in postnatal day 6 brain, when astrocytes are not yet mature. In contrast, granule cell precursors of the subgranular zone of adult hippocampus did undergo diurnal changes in Fabp7 expression. These changes paralleled oscillations in Fabp7 mRNA throughout the brain suggesting that cell-coordinated signals likely control brain-wide Fabp7 mRNA expression. Immunoblots revealed that Fabp7 protein levels also underwent diurnal changes in abundance, with peak levels occurring in the dark period. Of clock or clock-regulated genes, the synchronized, global cycling pattern of Fabp7 expression is unique and implicates glial cells in the response or modulation of activity and/or circadian rhythms.
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影响因子：
4.4
作者：
Gerstner, Jason R.;Landry, Charles F.
通讯作者：
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