Rapid and sensitive detection of an intracellular pathogen in human peripheral leukocytes with hybridizing magnetic relaxation nanosensors.
Rapid and sensitive detection of an intracellular pathogen in human peripheral leukocytes with hybridizing magnetic relaxation nanosensors.
复制标题
DOI：
10.1371/journal.pone.0035326
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Perez JM
中科院分区：
文献类型：
--
作者：
Kaittanis C;Boukhriss H;Santra S;Naser SA;Perez JM
细菌感染仍然是全球主要的医疗保健问题。快速、灵敏地检测导致这些感染的病原体将有助于正确诊断疾病并加快治疗。最重要的是细胞内生长缓慢的病原体，它们存在于外周白细胞内，逃避免疫系统的识别和传统培养方法的检测。在此，我们报告了使用杂交磁性纳米传感器（hMRS）来检测细胞内病原体鸟分枝杆菌。副结核病（MAP）。 hMRS 旨在与 MAP 基因组中发现的独特基因组序列结合，导致样本的磁共振信号发生显着变化。使用 hMRS 筛选临床相关样本，包括组织和血液，并将结果与传统 PCR 分析进行比较。在不到一个小时的时间内，hMRS 在实验室培养物、临床分离株、血液和匀浆组织库中鉴定出了 MAP 阳性样本。在疾病状态预测方面，hMRS 与培养方法的比较表明，hMRS 优于现有的培养方法，同时速度明显更快（1 小时 vs 12 周）。此外，使用一台仪器和一种纳米颗粒制剂，我们能够在基因组和表位水平上检测临床样本中的细胞内细菌靶标。总体而言，由于纳米颗粒在不同的环境环境中都很稳定，并且比 PCR 酶便宜得多，因此可以预见，hMRS 在临床和现场使用的潜在用途是，通过单一的、可部署的仪器在临床和复杂的环境样本中多重识别微生物病原体和其他疾病相关的生物标志物。
Bacterial infections are still a major global healthcare problem. The quick and sensitive detection of pathogens responsible for these infections would facilitate correct diagnosis of the disease and expedite treatment. Of major importance are intracellular slow-growing pathogens that reside within peripheral leukocytes, evading recognition by the immune system and detection by traditional culture methods. Herein, we report the use of hybridizing magnetic nanosensors (hMRS) for the detection of an intracellular pathogen, Mycobacterium avium spp. paratuberculosis (MAP). The hMRS are designed to bind to a unique genomic sequence found in the MAP genome, causing significant changes in the sample’s magnetic resonance signal. Clinically relevant samples, including tissue and blood, were screened with hMRS and results were compared with traditional PCR analysis. Within less than an hour, the hMRS identified MAP-positive samples in a library of laboratory cultures, clinical isolates, blood and homogenized tissues. Comparison of the hMRS with culture methods in terms of prediction of disease state revealed that the hMRS outperformed established culture methods, while being significantly faster (1 hour vs 12 weeks). Additionally, using a single instrument and one nanoparticle preparation we were able to detect the intracellular bacterial target in clinical samples at the genomic and epitope levels. Overall, since the nanoparticles are robust in diverse environmental settings and substantially more affordable than PCR enzymes, the potential clinical and field-based use of hMRS in the multiplexed identification of microbial pathogens and other disease-related biomarkers via a single, deployable instrument in clinical and complex environmental samples is foreseen.
登录
查看更多内容
影响因子：
36.8
作者：
CHIODINI, RJ
通讯作者：
CHIODINI, RJ
细菌程序性细胞死亡和细菌多细胞行为。
影响因子：
4.5
作者：
通讯作者：
--
影响因子：
11.2
作者：
Grimm, J;Perez, JM;Weissleder, R
通讯作者：
Weissleder, R
影响因子：
16.1
作者：
Kaittanis, Charalambos;Santra, Santimukul;Perez, J. Manuel
通讯作者：
Perez, J. Manuel
影响因子：
64.5
作者：
Kruse JP;Gu W
通讯作者：
Gu W