Dirhamnolipids secreted from Pseudomonas aeruginosa modify anjpegungal susceptibility of Aspergillus fumigatus by inhibiting β1,3 glucan synthase activity.
Dirhamnolipids secreted from Pseudomonas aeruginosa modify anjpegungal susceptibility of Aspergillus fumigatus by inhibiting β1,3 glucan synthase activity.
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DOI：
10.1038/ismej.2017.32
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发表时间：
2017-07
期刊：
影响因子：
--
通讯作者：
Beauvais A
中科院分区：
文献类型：
--
作者：
Briard B;Rasoldier V;Bomme P;ElAouad N;Guerreiro C;Chassagne P;Muszkieta L;Latgé JP;Mulard L;Beauvais A
铜绿假单胞菌和烟曲霉是导致囊性纤维化患者大多数慢性感染的两种微生物。已知铜绿假单胞菌在慢性感染期间产生群体感应控制的鼠李糖脂。在这里，我们表明，从铜绿假单胞菌分泌的二鼠李糖脂（i）诱导A。烟曲霉产生细胞外基质，富含半乳糖胺半乳聚糖，1，8-二羟基萘（DHN）-和脓黑色素，周围的菌丝，这有利于铜绿假单胞菌结合和（ii）抑制A。通过阻断β 1，3葡聚糖合成酶（GS）活性，从而改变细胞壁结构，抑制烟曲霉生长。A.在diRhls存在下，对烟曲霉的生长导致与用无刺棘白菌素处理后的生长表型相似的生长表型，显示多分支菌丝和富含几丁质的较厚细胞壁。diRhl与β 1，3-GS的相互作用主要是通过与脂酰基链相连的两个鼠李糖基团的diRhl结构实现的，但diRhl与β 1，3-GS的作用位点与棘白菌素不同，且与唑类化合物具有协同抗真菌作用。
Pseudomonas aeruginosa and Aspergillus fumigatus are the two microorganisms responsible for most of the chronic infections in cystic fibrosis patients. P. aeruginosa is known to produce quorum-sensing controlled rhamnolipids during chronic infections. Here we show that the dirhamnolipids secreted from P. aeruginosa (i) induce A. fumigatus to produce an extracellular matrix, rich in galactosaminogalactan, 1,8-dihydroxynaphthalene (DHN)- and pyo-melanin, surrounding their hyphae, which facilitates P. aeruginosa binding and (ii) inhibit A. fumigatus growth by blocking β1,3 glucan synthase (GS) activity, thus altering the cell wall architecture. A. fumigatus in the presence of diRhls resulted in a growth phenotype similar to that upon its treatment with anjpegungal echinocandins, showing multibranched hyphae and thicker cell wall rich in chitin. The diRhl structure containing two rhamnose moieties attached to fatty acyl chain is essential for the interaction with β1,3 GS; however, the site of action of diRhls on GS is different from that of echinocandins, and showed synergistic anjpegungal effect with azoles.
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影响因子：
3.6
作者：
Diggle, SP;Winzer, K;Williams, P
通讯作者：
Williams, P
影响因子：
6.7
作者：
Gresnigt MS;Bozza S;Becker KL;Joosten LA;Abdollahi-Roodsaz S;van der Berg WB;Dinarello CA;Netea MG;Fontaine T;De Luca A;Moretti S;Romani L;Latge JP;van de Veerdonk FL
通讯作者：
van de Veerdonk FL
对囊性纤维化中曲霉菌病不同表现的多国评估。
影响因子：
3.7
作者：
Armstead J;Morris J;Denning DW
通讯作者：
Denning DW
影响因子：
3.7
作者：
Bjarnsholt T;Jensen PØ;Jakobsen TH;Phipps R;Nielsen AK;Rybtke MT;Tolker-Nielsen T;Givskov M;Høiby N;Ciofu O;Scandinavian Cystic Fibrosis Study Consortium
通讯作者：
Scandinavian Cystic Fibrosis Study Consortium
影响因子：
5
作者：
Abdel-Mawgoud, Ahmad Mohammad;Lepine, Francois;Deziel, Eric
通讯作者：
Deziel, Eric