Multilocus Sequence Typing Reveals both Shared and Unique Genotypes of Cryptococcus neoformans in Jiangxi Province, China.
Multilocus Sequence Typing Reveals both Shared and Unique Genotypes of Cryptococcus neoformans in Jiangxi Province, China.
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多焦点序列键入揭示了中国江西省的加密赛车的共享和独特的基因型。
DOI：
10.1038/s41598-018-20054-4
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发表时间：
2018-01-24
影响因子：
4.6
通讯作者：
Hu LH
中科院分区：
文献类型：
--
作者：
Chen YH;Yu F;Bian ZY;Hong JM;Zhang N;Zhong QS;Hang YP;Xu J;Hu LH
隐球菌病是一种全球分布的传染性真菌疾病。然而，在世界许多地区，对其分子流行病学仍知之甚少。在这项研究中，我们分析了来自中国中南部江西省14个地区的86株临床新型隐球菌。每个分离株来自不同的患者，86例患者中有35例（40.7%）感染了HIV。所有菌株均为血清型A和交配型α (MATα)。基于7个核位点DNA序列的基因分型结果显示，86株分离株中存在8种序列类型（STs），其中包括2种未在世界其他地区报道的新型STs。ST5是优势基因型，我们的比较分析表明，江西的这些基因型可能起源于中国内外其他地区的传播和/或江西境内其他基因型的突变。虽然5种抗真菌药物（氟胞嘧啶、两性霉素B、氟康唑、伊曲康唑和伏立康唑）均不耐药，但即使在同一st的分离株中，其最低抑菌浓度也存在明显差异。我们的研究结果表明，应进行持续监测，以了解该地区和其他地区新型C.的变化动态。
Cryptococcosis is a globally distributed infectious fungal disease. However, much remains unknown about its molecular epidemiology in many parts of the world. In this study, we analyzed 86 clinical Cryptococcus neoformans isolates from 14 regions in Jiangxi Province in south central China. Each isolate was from a different patient and 35 of the 86 (40.7%) patients were infected with HIV. All strains belonged to serotype A and mating type α (MATα). Genotyping based on DNA sequences at seven nuclear loci revealed eight sequence types (STs) among the 86 isolates, including two novel STs that have not been reported from other parts of the world. ST5 was the dominant genotype and our comparative analyses showed that these genotypes in Jiangxi likely originated by dispersal from other regions within and outside of China and/or mutations from another genotype within Jiangxi. Though none of the isolates was resistant to the five tested antifungal drugs (flucytosine, amphotericin B, fluconazole, itraconazole, and voriconazole), obvious differences in their minimum inhibitory concentrations were observed, even among isolates of the same ST. Our results suggest that continuous monitoring should be conducted to understand the changing dynamics of C. neoformans in this and other regions.
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影响因子：
3.2
作者：
Choi YH;Ngamskulrungroj P;Varma A;Sionov E;Hwang SM;Carriconde F;Meyer W;Litvintseva AP;Lee WG;Shin JH;Kim EC;Lee KW;Choi TY;Lee YS;Kwon-Chung KJ
通讯作者：
Kwon-Chung KJ
影响因子：
3.7
作者：
Hatthakaroon C;Pharkjaksu S;Chongtrakool P;Suwannakarn K;Kiratisin P;Ngamskulrungroj P
通讯作者：
Ngamskulrungroj P
全球范围内基于 MLST 的群体遗传分析揭示了新型隐球菌变种之间的克隆性。 grubii VNI 从巴西东南部的 HIV 患者中分离出来。
影响因子：
3.8
作者：
Ferreira-Paim K;Andrade-Silva L;Fonseca FM;Ferreira TB;Mora DJ;Andrade-Silva J;Khan A;Dao A;Reis EC;Almeida MT;Maltos A;Junior VR;Trilles L;Rickerts V;Chindamporn A;Sykes JE;Cogliati M;Nielsen K;Boekhout T;Fisher M;Kwon-Chung J;Engelthaler DM;Lazéra M;Meyer W;Silva-Vergara ML
通讯作者：
Silva-Vergara ML
影响因子：
9.4
作者：
McTaggart, Lisa R.;Lei, Eric;Zhang, Sean X.
通讯作者：
Zhang, Sean X.
影响因子：
3.2
作者：
Bolano, Alessandro;Stinchi, Silvia;Cardinali, Gianluigi
通讯作者：
Cardinali, Gianluigi