The Mulberry SPL Gene Family and the Response of MnSPL7 to Silkworm Herbivory through Activating the Transcription of MnTT2L2 in the Catechin Biosynthesis Pathway.
The Mulberry SPL Gene Family and the Response of MnSPL7 to Silkworm Herbivory through Activating the Transcription of MnTT2L2 in the Catechin Biosynthesis Pathway.
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DOI：
10.3390/ijms23031141
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发表时间：
2022-01-20
影响因子：
5.6
通讯作者：
He N
中科院分区：
文献类型：
--
作者：
Li H;Ma B;Luo Y;Wei W;Yuan J;Zhai C;He N
类鳞状细胞启动子结合蛋白（SPL）基因作为植物特有的转录因子，在植物发育调控和逆境适应中发挥重要作用。桑葚是一种重要的经济树种，但对SPL基因的系统研究却很少。在本研究中，我们在桑葚基因组中鉴定了15个全长SPL基因，分布在4条染色体上。系统发育分析将5种植物的SPL基因聚在一起，它们分别是苹果（Malus × aparticaBorkh）、大果杨（Populus sputicarpa）、山杨（M. notabilis、拟南芥（Arabidopsis thaliana）和水稻（Oryza sativa）分成五组。两个锌指（Zn1和Zn2）被发现在所有的MnSPLs的保守SBP结构域。基因结构和保守基序的比较分析显示，一组内的MnSPLs的保守性，而有显着的结构差异组之间。基因定量分析表明，MnSPLs的表达具有组织特异性，且MnSPLs在较老桑葚中表达量较高。此外，转录组数据显示，MnSPL7和MnSPL14的表达水平在家蚕植食性下显著增加。分子生物学实验表明，MnSPL7通过促进MnTT2L2的转录并进一步上调儿茶素合成基因（F3′H、DFR和LAR）的表达水平来响应植物取食。
SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes, as unique plant transcription factors, play important roles in plant developmental regulation and stress response adaptation. Although mulberry is a commercially valuable tree species, there have been few systematic studies on SPL genes. In this work, we identified 15 full-length SPL genes in the mulberry genome, which were distributed on 4 Morus notabilis chromosomes. Phylogenetic analysis clustered the SPL genes from five plants (Malus × domestica Borkh, Populus trichocarpa, M. notabilis, Arabidopsis thaliana, and Oryza sativa) into five groups. Two zinc fingers (Zn1 and Zn2) were found in the conserved SBP domain in all of the MnSPLs. Comparative analyses of gene structures and conserved motifs revealed the conservation of MnSPLs within a group, whereas there were significant structure differences among groups. Gene quantitative analysis showed that the expression of MnSPLs had tissue specificity, and MnSPLs had much higher expression levels in older mulberry leaves. Furthermore, transcriptome data showed that the expression levels of MnSPL7 and MnSPL14 were significantly increased under silkworm herbivory. Molecular experiments revealed that MnSPL7 responded to herbivory treatment through promoting the transcription of MnTT2L2 and further upregulating the expression levels of catechin synthesis genes (F3′H, DFR, and LAR).
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影响因子：
4.5
作者：
He J;Xu M;Willmann MR;McCormick K;Hu T;Yang L;Starker CG;Voytas DF;Meyers BC;Poethig RS
通讯作者：
Poethig RS
小麦 (Triticum aestivum L.) 中 SBP-box 基因家族的全基因组鉴定、表征和表达模式分析
影响因子：
4.6
作者：
Li Y;Song Q;Zhang Y;Li Z;Guo J;Chen X;Zhang G
通讯作者：
Zhang G
DOI：
10.3390/molecules23010004
发表时间：
2017-12-21
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Chen H;Yu W;Chen G;Meng S;Xiang Z;He N
通讯作者：
He N
影响因子：
27.5
作者：
Chao, Lu-Men;Liu, Yao-Qian;Chen, Xiao-Ya
通讯作者：
Chen, Xiao-Ya
影响因子：
16.6
作者：
Mao YB;Liu YQ;Chen DY;Chen FY;Fang X;Hong GJ;Wang LJ;Wang JW;Chen XY
通讯作者：
Chen XY