Antinociceptive and Antibacterial Properties of Anthocyanins and Flavonols from Fruits of Black and Non-Black Mulberries.
Antinociceptive and Antibacterial Properties of Anthocyanins and Flavonols from Fruits of Black and Non-Black Mulberries.
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DOI：
10.3390/molecules23010004
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发表时间：
2017-12-21
期刊：
影响因子：
--
通讯作者：
He N
中科院分区：
文献类型：
--
作者：
Chen H;Yu W;Chen G;Meng S;Xiang Z;He N
花青素和黄酮类是水果着色的重要色素。桑果含有丰富的花青素和黄酮醇，在传统中药中具有多种用途。研究了黑桑总黄酮(MnTF)、非黑桑总黄酮(MmTF)和珍珠白(Morus alba‘珍珠白’)果实总黄酮(Tf)的抗伤害和抗菌活性。经超高效液相色谱-可调谐紫外单四极杆检测(UPLC-TUV/QDA)鉴定，MnTF富含花色苷(11.3 mg/g)和黄酮醇(0.7 mg/g)。相比之下，MmTf和Maztf的黄酮醇含量较低，Maztf不含花色苷。MnTf对大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌的抗伤害性和抗菌活性显著高于MmTf和MazTf。MnTf抑制IL-6、诱导型一氧化氮合酶(INOS)、磷酸化p65(p-p65)和磷酸化-IκBα(p-IκBα)的表达，升高IL-10的表达。此外，小鼠实验表明，氰基-3-O-葡萄糖苷(C3G)、芦丁(Ru)和异槲皮素(IQ)是抗伤害过程中的主要活性成分。其较强的抗伤害性作用与其较高的花青素和黄酮醇含量以及对促炎细胞因子、诱导型一氧化氮合酶和核因子-κB(NF-κB)途径相关蛋白的抑制作用有关。
Anthocyanins and flavones are important pigments responsible for the coloration of fruits. Mulberry fruit is rich in anthocyanins and flavonols, which have multiple uses in traditional Chinese medicine. The antinociceptive and antibacterial activities of total flavonoids (TF) from black mulberry (MnTF, TF of Morus nigra) and non-black mulberry (MmTF, TF of Morus mongolica; and MazTF, TF of Morus alba ‘Zhenzhubai’) fruits were studied. MnTF was rich in anthocyanins (11.3 mg/g) and flavonols (0.7 mg/g) identified by ultra-performance liquid chromatography–tunable ultraviolet/mass single-quadrupole detection (UPLC–TUV/QDa). Comparatively, MmTF and MazTF had low flavonol contents and MazTF had no anthocyanins. MnTF showed significantly higher antinociceptive and antibacterial activities toward Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus than MmTF and MazTF. MnTF inhibited the expression of interleukin 6 (IL-6), inducible nitric oxide synthase (iNOS), phospho-p65 (p-p65) and phospho-IκBα (p-IκBα), and increased interleukin 10 (IL-10). Additionally, mice tests showed that cyanidin-3-O-glucoside (C3G), rutin (Ru) and isoquercetin (IQ) were the main active ingredients in the antinociceptive process. Stronger antinociceptive effect of MnTF was correlated with its high content of anthocyanins and flavonols and its inhibitory effects on proinflammatory cytokines, iNOS and nuclear factor-κB (NF-κB) pathway-related proteins.
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影响因子：
11.2
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影响因子：
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通讯作者：
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