Alternative topogenesis of Mgm1 and mitochondrial morphology depend on ATP and a functional import motor.
Alternative topogenesis of Mgm1 and mitochondrial morphology depend on ATP and a functional import motor.
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DOI：
10.1083/jcb.200403022
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发表时间：
2004-04-26
期刊：
影响因子：
--
通讯作者：
Reichert AS
中科院分区：
文献类型：
--
作者：
Herlan M;Bornhövd C;Hell K;Neupert W;Reichert AS
酵母线粒体形态和线粒体DNA的遗传依赖于动力蛋白样GTdR Mgm 1。它以两种亚型存在于线粒体的膜间隙中，这两种亚型都是Mgm 1功能所需的。线粒体菱形蛋白酶Pcp 1/Rbd 1对大同种型的有限蛋白水解产生Mgm 1的短同种型，但这是如何调节的尚不清楚。我们发现，在其NH 2末端附近的Mgm 1包含两个保守的疏水性片段，其中更多的COOH-末端之一是由Pcp 1裂解。改变NH 2-末端片段的疏水性调节了亚型的比例，并导致线粒体片段化。Mgm 1的短同种型和线粒体形态的形成进一步依赖于功能性蛋白质输入马达和基质中的ATP水平。我们的数据表明，一种新的途径，我们称之为替代地形，代表了一个关键的监管机制，确保两个Mgm 1亚型的平衡形成。线粒体ATP水平可能通过这一过程控制线粒体形态。
Mitochondrial morphology and inheritance of mitochondrial DNA in yeast depend on the dynamin-like GTPase Mgm1. It is present in two isoforms in the intermembrane space of mitochondria both of which are required for Mgm1 function. Limited proteolysis of the large isoform by the mitochondrial rhomboid protease Pcp1/Rbd1 generates the short isoform of Mgm1 but how this is regulated is unclear. We show that near its NH2 terminus Mgm1 contains two conserved hydrophobic segments of which the more COOH-terminal one is cleaved by Pcp1. Changing the hydrophobicity of the NH2-terminal segment modulated the ratio of the isoforms and led to fragmentation of mitochondria. Formation of the short isoform of Mgm1 and mitochondrial morphology further depend on a functional protein import motor and on the ATP level in the matrix. Our data show that a novel pathway, to which we refer as alternative topogenesis, represents a key regulatory mechanism ensuring the balanced formation of both Mgm1 isoforms. Through this process the mitochondrial ATP level might control mitochondrial morphology.
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DOI：
10.1111/j.1432-1033.1976.tb10769.x
发表时间：
1976-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
FOURY, F;TZAGOLOFF, A
通讯作者：
TZAGOLOFF, A
影响因子：
11.4
作者：
Mokranjac, D;Sichting, M;Hell, K
通讯作者：
Hell, K
影响因子：
64.8
作者：
Sirrenberg, C;Bauer, MF;Brunner, M
通讯作者：
Brunner, M
影响因子：
2.5
作者：
GUAN, KL;FARH, L;DESCHENES, RJ
通讯作者：
DESCHENES, RJ
影响因子：
7.8
作者：
Wong, E D;Wagner, J A;Gorsich, S W;McCaffery, J M;Shaw, J M;Nunnari, J
通讯作者：
Nunnari, J