Gunn rats with glial activation in the hippocampus show prolonged immobility time in the forced swimming test and tail suspension test.
Gunn rats with glial activation in the hippocampus show prolonged immobility time in the forced swimming test and tail suspension test.
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DOI：
10.1002/brb3.1028
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发表时间：
2018-08
影响因子：
3.1
通讯作者：
Horiguchi J
中科院分区：
文献类型：
--
作者：
Arauchi R;Hashioka S;Tsuchie K;Miyaoka T;Tsumori T;Limoa E;Azis IA;Oh-Nishi A;Miura S;Otsuki K;Kanayama M;Izuhara M;Nagahama M;Kawano K;Araki T;Liaury K;Abdullah RA;Wake R;Hayashida M;Inoue K;Horiguchi J
最近的研究表明，神经胶质细胞活化在精神疾病的发病机制中起作用，如精神分裂症和抑郁症。我们先前证明，患有高胆红素血症的古恩大鼠表现出先天性神经胶质增生和精神分裂症样行为。由于已经表明重度抑郁症涉及与神经炎症相关的胶质细胞活化，我们使用强迫游泳试验（FST）和悬尾试验（TST）检查了古恩大鼠是否表现出抑郁样行为。此外，我们使用小胶质细胞标志物离子钙结合衔接分子（Iba）1和星形胶质细胞标志物S100 B的免疫组织化学分析定量评价了古恩大鼠海马中的小胶质细胞增生和星形胶质细胞增生。FST和TST均显示古恩大鼠的不动时间显著长于正常对照Wistar大鼠，表明古恩大鼠有些无助，是抑郁样行为的迹象。在免疫组织化学定量分析中，与Wistar大鼠相比，古恩大鼠齿状回（DG）、海马角（CA）1和CA 3中的Iba 1免疫反应性以及CA 1和CA 3中的Iba 1阳性细胞数量显著增加。与Wistar大鼠相比，古恩大鼠DG、CA 1和CA 3中的S100 B免疫反应性以及DG和CA 3中的S100 B阳性细胞数量显著增加。我们的研究结果表明，小胶质细胞和星形胶质细胞激活的古恩大鼠和他们的习得性无助可能与胶质细胞的激活。
Recent studies imply that glial activation plays a role in the pathogenesis of psychiatric disorders, such as schizophrenia and major depression. We previously demonstrated that Gunn rats with hyperbilirubinemia show congenital gliosis and schizophrenia‐like behavior. As it has been suggested that major depression involves glial activation associated with neuroinflammation, we examined whether Gunn rats show depression‐like behavior using the forced swimming test (FST) and the tail suspension test (TST). In addition, we quantitatively evaluated both microgliosis and astrogliosis in the hippocampus of Gunn rats using immunohistochemistry analysis of the microglial marker ionized calcium‐binding adaptor molecule (Iba) 1 and the astrocytic marker S100B. Both the FST and TST showed that immobility time of Gunn rats was significantly longer than that of normal control Wistar rats, indicating that Gunn rats are somewhat helpless, a sign of depression‐like behavior. In the quantification of immunohistochemical analysis, Iba1immunoreactivity in the dentate gyrus (DG), cornu ammonis (CA) 1, and CA3 and the number of Iba1‐positive cells in the CA1 and CA3 were significantly increased in Gunn rats compared to Wistar rats. S100B immunoreactivity in the DG, CA1, and CA3 and the number of S100B‐positive cells in the DG and CA3 were significantly increased in Gunn rats compared to Wistar rats. Our findings suggest that both microglia and astrocyte are activated in Gunn rats and their learned helplessness could be related to glial activation.
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影响因子：
4.6
作者：
Biesmans S;Meert TF;Bouwknecht JA;Acton PD;Davoodi N;De Haes P;Kuijlaars J;Langlois X;Matthews LJ;Ver Donck L;Hellings N;Nuydens R
通讯作者：
Nuydens R
影响因子：
2.5
作者：
Bayer, TA;Buslei, R;Falkai, P
通讯作者：
Falkai, P
影响因子：
6.2
作者：
Fernandes, Adelaide;Barateiro, Andreia;Brites, Dora
通讯作者：
Brites, Dora
影响因子：
4.2
作者：
Hashioka, Sadayuki;Klegeris, Andis;McGeer, Patrick L.
通讯作者：
McGeer, Patrick L.
影响因子：
6.2
作者：
Norden DM;Trojanowski PJ;Villanueva E;Navarro E;Godbout JP
通讯作者：
Godbout JP