Proteomic Studies of the Biofilm Matrix including Outer Membrane Vesicles of Burkholderia multivorans C1576, a Strain of Clinical Importance for Cystic Fibrosis.
Proteomic Studies of the Biofilm Matrix including Outer Membrane Vesicles of Burkholderia multivorans C1576, a Strain of Clinical Importance for Cystic Fibrosis.
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DOI：
10.3390/microorganisms8111826
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发表时间：
2020-11-19
期刊：
影响因子：
4.5
通讯作者：
Sblattero D
中科院分区：
文献类型：
--
作者：
Terán LC;Distefano M;Bellich B;Petrosino S;Bertoncin P;Cescutti P;Sblattero D
生物膜是包裹在高度水合基质中的微生物细胞的聚集体，该基质由自身产生的胞外聚合物(EPS)组成，EPS由多糖、蛋白质、核酸和脂类组成。尽管生物膜基质多糖已被揭开，但对基质相关蛋白的特性和功能仍知之甚少。通过这项工作，我们进行了一种全面的蛋白质组学方法来揭示与生物被膜生长的多发性伯克霍尔德氏菌C1576参考菌株(囊性纤维化临床分离株)的基质相关的蛋白质的身份。透射电子显微镜显示，多食杆菌C1576还释放生物膜基质中的外膜小泡(OMV)，就像其他革兰氏阴性菌已经证明的那样。蛋白质组学分析表明，细胞质和膜结合蛋白在基质中广泛存在，而OMV高度富含外膜蛋白和铁载体。我们的数据表明，细胞裂解和OMVS的产生是多食杆菌C1576生物膜基质最重要的蛋白质来源。值得注意的是，一些已鉴定的蛋白质是裂解酶、铁载体和参与清除活性氧簇(ROS)的蛋白质。这些蛋白可能有助于多食杆菌C1576在宿主组织入侵和防御免疫系统攻击方面的作用。
Biofilms are aggregates of microbial cells encased in a highly hydrated matrix made up of self-produced extracellular polymeric substances (EPS) which consist of polysaccharides, proteins, nucleic acids, and lipids. While biofilm matrix polysaccharides are unraveled, there is still poor knowledge about the identity and function of matrix-associated proteins. With this work, we performed a comprehensive proteomic approach to disclose the identity of proteins associated with the matrix of biofilm-growing Burkholderia multivorans C1576 reference strain, a cystic fibrosis clinical isolate. Transmission electron microscopy showed that B. multivorans C1576 also releases outer membrane vesicles (OMVs) in the biofilm matrix, as already demonstrated for other Gram-negative species. The proteomic analysis revealed that cytoplasmic and membrane-bound proteins are widely represented in the matrix, while OMVs are highly enriched in outer membrane proteins and siderophores. Our data suggest that cell lysis and OMVs production are the most important sources of proteins for the B. multivorans C1576 biofilm matrix. Of note, some of the identified proteins are lytic enzymes, siderophores, and proteins involved in reactive oxygen species (ROS) scavenging. These proteins might help B. multivorans C1576 in host tissue invasion and defense towards immune system assaults.
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影响因子：
3.7
作者：
Cuzzi B;Herasimenka Y;Silipo A;Lanzetta R;Liut G;Rizzo R;Cescutti P
通讯作者：
Cescutti P
影响因子：
2.1
作者：
Ganz, Tomas
通讯作者：
Ganz, Tomas
影响因子：
3.6
作者：
Allesen-Holm, M;Barken, KB;Tolker-Nielsen, T
通讯作者：
Tolker-Nielsen, T
影响因子：
4.4
作者：
Harmsen, Morten;Lappann, Martin;Molin, Soren
通讯作者：
Molin, Soren
DOI：
10.1099/mic.0.036848-0
发表时间：
2010-07
期刊：
Microbiology (Reading, England)
影响因子：
--
作者：
Bakkal S;Robinson SM;Ordonez CL;Waltz DA;Riley MA
通讯作者：
Riley MA