Role of bacteriocins in mediating interactions of bacterial isolates taken from cystic fibrosis patients.
Role of bacteriocins in mediating interactions of bacterial isolates taken from cystic fibrosis patients.
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DOI：
10.1099/mic.0.036848-0
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发表时间：
2010-07
期刊：
影响因子：
--
通讯作者：
Riley MA
中科院分区：
文献类型：
--
作者：
Bakkal S;Robinson SM;Ordonez CL;Waltz DA;Riley MA
铜绿假单胞菌（Pa）和洋葱伯克霍尔德菌复合体（Bcc）肺部感染是导致囊性纤维化（CF）死亡的主要原因。然而，人们对这两种经常共同感染的物种之间的生态相互作用知之甚少。这项研究提供了被认为是CF患者恢复的Pa和Bcc菌株的种内和种间细菌素样抑制潜力的第一份报告。共筛选出66株具有细菌素样抑制活性的菌株（97%的Pa菌株和68%的Bcc菌株具有抑制活性），其中大部分具有跨物种作用。进一步的表型和分子基础的分析显示，这种抑制的来源是不同的两个物种。在Pa中，大部分抑制活性是由于众所周知的S和RF脓毒杆菌。相比之下，Bcc抑制是由于未知的机制，尽管rf样毒素涉及一些菌株。这些数据表明，基于细菌素的抑制可能在控制CF肺中Pa和Bcc的相互作用中发挥作用，因此可能为介导这些通常致命的感染提供一种新的方法。
Pseudomonas aeruginosa (Pa) and Burkholderia cepacia complex (Bcc) lung infections are responsible for much of the mortality in cystic fibrosis (CF). However, little is known about the ecological interactions between these two, often co-infecting, species. This study provides what is believed to be the first report of the intra- and interspecies bacteriocin-like inhibition potential of Pa and Bcc strains recovered from CF patients. A total of 66 strains were screened, and shown to possess bacteriocin-like inhibitory activity (97 % of Pa strains and 68 % of Bcc strains showed inhibitory activity), much of which acted across species boundaries. Further phenotypic and molecular-based assays revealed that the source of this inhibition differs for the two species. In Pa, much of the inhibitory activity is due to the well-known S and RF pyocins. In contrast, Bcc inhibition is due to unknown mechanisms, although RF-like toxins were implicated in some strains. These data suggest that bacteriocin-based inhibition may play a role in governing Pa and Bcc interactions in the CF lung and may, therefore, offer a novel approach to mediating these often fatal infections.
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