Versatility of the Burkholderia cepacia complex for the biosynthesis of exopolysaccharides: a comparative structural investigation.
Versatility of the Burkholderia cepacia complex for the biosynthesis of exopolysaccharides: a comparative structural investigation.
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DOI：
10.1371/journal.pone.0094372
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Cescutti P
中科院分区：
文献类型：
--
作者：
Cuzzi B;Herasimenka Y;Silipo A;Lanzetta R;Liut G;Rizzo R;Cescutti P
洋葱伯克霍尔德菌复合体聚集了至少十八种自然界中普遍存在的密切相关的物种。一些分离株显示出生物防治、生物修复和促进植物生长的有益潜力。相反，其他菌株是植物和免疫功能低下个体（如囊性纤维化患者）的病原体。在这些受试者中，它们可能导致呼吸道感染，有时会导致致命的后果。大多数洋葱伯克霍尔德菌复合体在富含甘露醇的培养基上生长时呈粘液状，并且它们还形成生物膜，这是两个相关的特征，因为多糖是生物膜基质的重要组成部分。此外，多糖通过抑制中性粒细胞趋化性和抗菌肽活性以及清除活性氧，有助于细菌在恶劣环境中生存。这些微生物产生具有不同结构的胞外多糖的能力已被文献中的大量文章所证明。然而，人们对生物膜中产生的多糖类型及其与非生物膜条件下获得的多糖的关系知之甚少。本研究的目的是确定九种洋葱伯克霍尔德菌复合体产生的胞外多糖的类型。然后选择两个分离物来比较琼脂平板上产生的多糖与纤维素膜上形成的生物膜中形成的多糖。该研究是使用核磁共振波谱、高效尺寸排阻色谱以及气相色谱与质谱联用进行的。结果表明，该复合物能够产生多种胞外多糖，最常见的是混合物，并且最常见的胞外多糖始终是洋葱胞外多糖。此外，还确定了两种新的多糖结构：一种由甘露糖和鼠李糖组成，另一种含有半乳糖和葡萄糖醛酸。从琼脂平板上的培养物中获得的胞外多糖与从纤维素膜上的生物膜中提取的胞外多糖的比较显示出重要的差异，因此表明从非生物膜条件外推的数据可能并不总是适用。
The Burkholderia cepacia Complex assembles at least eighteen closely related species that are ubiquitous in nature. Some isolates show beneficial potential for biocontrol, bioremediation and plant growth promotion. On the contrary, other strains are pathogens for plants and immunocompromised individuals, like cystic fibrosis patients. In these subjects, they can cause respiratory tract infections sometimes characterised by fatal outcome. Most of the Burkholderia cepacia Complex species are mucoid when grown on a mannitol rich medium and they also form biofilms, two related characteristics, since polysaccharides are important component of biofilm matrices. Moreover, polysaccharides contribute to bacterial survival in a hostile environment by inhibiting both neutrophils chemotaxis and antimicrobial peptides activity, and by scavenging reactive oxygen species. The ability of these microorganisms to produce exopolysaccharides with different structures is testified by numerous articles in the literature. However, little is known about the type of polysaccharides produced in biofilms and their relationship with those obtained in non-biofilm conditions. The aim of this study was to define the type of exopolysaccharides produced by nine species of the Burkholderia cepacia Complex. Two isolates were then selected to compare the polysaccharides produced on agar plates with those formed in biofilms developed on cellulose membranes. The investigation was conducted using NMR spectroscopy, high performance size exclusion chromatography, and gas chromatography coupled to mass spectrometry. The results showed that the Complex is capable of producing a variety of exopolysaccharides, most often in mixture, and that the most common exopolysaccharide is always cepacian. In addition, two novel polysaccharide structures were determined: one composed of mannose and rhamnose and another containing galactose and glucuronic acid. Comparison of exopolysaccharides obtained from cultures on agar plates with those extracted from biofilms on cellulose membranes showed important differences, thus suggesting that extrapolating data from non-biofilm conditions might not always be applicable.
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影响因子：
11.3
作者：
Mann EE;Wozniak DJ
通讯作者：
Wozniak DJ
影响因子：
2.1
作者：
Lagatolla, C;Skerlavaj, S;Cescutti, P
通讯作者：
Cescutti, P
影响因子：
3.1
作者：
ALBERSHEIM, PETER;NEVINS, DONALD J.;KARR, ARTHUR
通讯作者：
KARR, ARTHUR
影响因子：
3.1
作者：
HARRIS, PJ;HENRY, RJ;STONE, BA
通讯作者：
STONE, BA
DOI：
10.1016/j.jcf.2004.04.004
发表时间：
2004-08-01
期刊：
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
影响因子：
--
作者：
Chiarini, Luigi;Cescutti, Paola;Rizzo, Roberto
通讯作者：
Rizzo, Roberto