A new oligonucleotide microarray for detection of pathogenic and non-pathogenic Legionella spp.
A new oligonucleotide microarray for detection of pathogenic and non-pathogenic Legionella spp.
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DOI：
10.1371/journal.pone.0113863
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Cao B;Liu X;Yu X;Chen M;Feng L;Wang L
自从军团菌这种致命疾病被发现以来，嗜肺军团菌一直被认为是军团菌病的主要病因。除嗜肺乳杆菌外，军团菌后来被发现是许多非嗜肺乳杆菌感染的原因。构造。由于缺乏有效的诊断，嗜肺菌感染可能未被发现。本文对10个军团菌种和亚种的16S-23S rRNA基因转录间隔序列（ITS）进行了测序，包括L. anisa、L. bozemanii、L. dumoffii、L. fairfield densis、L. gormanii、L. jordanis、L. maceachernii、L. micdadei、L. pneumophila亚种。嗜肺乳杆菌亚种。并据此开发了一种快速寡核苷酸芯片检测技术，鉴定出12种最常见的军团菌，包括L. anisa、L. bozemanii、L. dumoffii、L. gormanii、L. jordanis、L. longbehae、L. maceachernii、L. micdadei和L. pneumophila（包括亚种）11种致病种。瞭解,subspp。Fraseri和subspp。一个非致病性的种，L. fairfieldensis。29个探针可重复检测多种军团菌，具有高特异性。利用52株菌株，包括30株靶菌和22株非靶菌，对寡核苷酸芯片检测进行验证。基因组DNA检测灵敏度为1.0 ng，军团菌培养物检测灵敏度为13 CFU/100 mL。该芯片检测了7个空调冷凝水样本，准确率为100%，验证了该技术在基础微生物学、临床诊断、食品安全和流行病学监测方面的应用前景。基于ITS的系统发育研究还表明，非致病性费尔菲尔德致密乳杆菌与其他9种致病性军团菌最接近嗜肺乳杆菌。
Legionella pneumophila has been recognized as the major cause of legionellosis since the discovery of the deadly disease. Legionella spp. other than L. pneumophila were later found to be responsible to many non-pneumophila infections. The non-L. pneumophila infections are likely under-detected because of a lack of effective diagnosis. In this report, we have sequenced the 16S-23S rRNA gene internal transcribed spacer (ITS) of 10 Legionella species and subspecies, including L. anisa, L. bozemanii, L. dumoffii, L. fairfieldensis, L. gormanii, L. jordanis, L. maceachernii, L. micdadei, L. pneumophila subspp. fraseri and L. pneumophila subspp. pasculleii, and developed a rapid oligonucleotide microarray detection technique accordingly to identify 12 most common Legionella spp., which consist of 11 pathogenic species of L. anisa, L. bozemanii, L. dumoffii, L. gormanii, L. jordanis, L. longbeachae, L. maceachernii, L. micdadei, and L. pneumophila (including subspp. pneumophila, subspp. fraseri, and subspp. pasculleii) and one non-pathogenic species, L. fairfieldensis. Twenty-nine probes that reproducibly detected multiple Legionella species with high specificity were included in the array. A total of 52 strains, including 30 target pathogens and 22 non-target bacteria, were used to verify the oligonucleotide microarray assay. The sensitivity of the detection was at 1.0 ng with genomic DNA or 13 CFU/100 mL with Legionella cultures. The microarray detected seven samples of air conditioner-condensed water with 100% accuracy, validating the technique as a promising method for applications in basic microbiology, clinical diagnosis, food safety, and epidemiological surveillance. The phylogenetic study based on the ITS has also revealed that the non-pathogenic L. fairfieldensis is the closest to L. pneumophila than the nine other pathogenic Legionella spp.
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开发用于检测和鉴定嗜肺军团菌所有 15 种不同 O 抗原形式的 DNA 微阵列方法
影响因子：
4.4
作者：
Cao, Boyang;Yao, Fangfang;Wang, Lei
通讯作者：
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影响因子：
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作者：
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作者：
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影响因子：
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作者：
CIANCIOTTO, NP;EISENSTEIN, BI;ENGLEBERG, NC
通讯作者：
ENGLEBERG, NC
影响因子：
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作者：
Gürtler, V
通讯作者：
Gürtler, V