Optical assessment of changes in mechanical and chemical properties of adipose tissue in diet-induced obese rats.
Optical assessment of changes in mechanical and chemical properties of adipose tissue in diet-induced obese rats.
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DOI：
10.1002/jbio.201600281
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发表时间：
2017-12
影响因子：
2.8
通讯作者：
Yakovlev VV
中科院分区：
文献类型：
--
作者：
Troyanova-Wood M;Gobbell C;Meng Z;Gashev AA;Yakovlev VV
肥胖正在成为世界范围内健康问题的主要原因。肥胖和超重与组织的结构和化学变化有关；然而，几乎没有方法可以同时测量这些变化。利用布里渊和拉曼显微光谱，可以同时评估机械和化学差异。我们假设布里渊光谱学可以测量脂肪组织的硬度，脂肪组织的硬度在肥胖中增加。对对照组和饮食诱导肥胖成年大鼠的棕色和白色脂肪组织进行了分析。结果表明，肥胖组的两种脂肪组织的高频纵向弹性模数均大于对照组，且棕色脂肪普遍比白色脂肪僵硬。拉曼光谱显示脂肪组织中脂质的积聚超过了纤维化的速度，高脂饮食对棕色脂肪的影响大于白色脂肪。总体而言，布里渊和拉曼显微光谱仪的强大组合首次成功地同时评估了脂肪组织的机械性能和化学成分。结果表明，肥胖导致脂肪组织的硬度和脂肪含量增加，与白色脂肪组织相比，棕色脂肪组织经历了更明显的变化。肥胖与组织的结构和化学变化有关；然而，很少有方法能够同时测量它们。利用布里渊和拉曼显微光谱的强大组合，首次同时评估了对照组和肥胖大鼠之间脂肪组织的机械和化学差异。结果表明，肥胖导致脂肪组织的硬度和脂肪含量增加，其中棕色脂肪的变化比白色脂肪大。
Obesity is becoming a leading cause of health problems world-wide. Obesity and overweight are associated with the structural and chemical changes in tissues; however, few methods exist that allow for concurrent measurement of these changes. Using Brillouin and Raman microspectroscopy, both the mechanical and chemical differences can be assessed simultaneously. We hypothesized that Brillouin spectroscopy can measure the adipose tissues’ stiffness, which increases in obesity. Samples of brown and white adipose tissues obtained from control and diet-induced obese adult rats were analyzed. The results show that both adipose tissues of the obese group exhibit a greater high-frequency longitudinal elastic modulus than the control samples, and that the brown fat is generally stiffer than white adipose. The Raman spectra indicates that the lipids’ accumulation in adipose tissue outpaces the fibrosis, and that the high-fat diet has a greater effect on the brown adipose than the white fat. Overall, the powerful combination of Brillouin and Raman microspectroscopies successfully assessed both the mechanical properties and chemical composition of adipose tissue simultaneously for the first time. The results indicate that the adipose tissue experiences an obesity-induced increase in stiffness and lipid content, with the brown adipose tissue undergoing a more pronounced change compared to white adipose. Obesity is associated with tissues’ structural and chemical changes; however, few methods are capable of their concurrent measurement. Using a powerful combination of Brillouin and Raman microspectroscopy, adipose tissue’s mechanical and chemical differences between control and obese rats are assessed simultaneously for the first time. The results indicate the adipose tissue experiences an obesity-induced increase in stiffness and lipid content, with brown adipose undergoing a greater change than white adipose.
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影响因子：
1.6
作者：
Giarola, M.;Guella, G.;Sbarbati, A.
通讯作者：
Sbarbati, A.
影响因子：
2.8
作者：
Meng, Zhaokai;Lopez, Sandra C. Bustamante;Yakovlev, Vladislav V.
通讯作者：
Yakovlev, Vladislav V.
影响因子：
4
作者：
Antonacci, Giuseppe;Foreman, Matthew R.;Toeroek, Peter
通讯作者：
Toeroek, Peter
DOI：
10.1039/c5an01700a
发表时间：
2015-11-07
期刊：
The Analyst
影响因子：
--
作者：
Meng Z;Petrov GI;Yakovlev VV
通讯作者：
Yakovlev VV
影响因子：
2.4
作者：
Bolognini, Gabriele;Soto, Marcelo A.;Di Pasquale, Fabrizio
通讯作者：
Di Pasquale, Fabrizio