Reliability of transcutaneous measurement of renal function in various strains of conscious mice.
Reliability of transcutaneous measurement of renal function in various strains of conscious mice.
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DOI：
10.1371/journal.pone.0071519
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Melk A
中科院分区：
文献类型：
--
作者：
Schock-Kusch D;Geraci S;Ermeling E;Shulhevich Y;Sticht C;Hesser J;Stsepankou D;Neudecker S;Pill J;Schmitt R;Melk A
使用血液和/或尿液采样来测量实验室动物的肾功能不仅是劳动密集型的，而且还会给动物带来压力。已经开发了几种基于荧光的经皮测量实验室动物肾小球滤过率（GFR）的方法。它们允许基于荧光外源性标记物的消除动力学来测量GFR。这些研究均未涉及相同动物中测量值的重现性。因此，在本研究中，在清醒小鼠中使用荧光肾脏标记物FITC-Sinistrin研究了经皮GFR评估方法的重现性。我们在三天内对五组小鼠（Balb/c、C57 BL/6、SV 129、3-4月龄的NMRI和一组24月龄的C57 BL/6）进行了两次经皮GFR测量。评价了GFR的日间重现性、品系内和品系间变异性以及年龄对这些参数的影响。随后两次GFR测量之间未检测到显著差异。在Balb/c中检测到FITC-Sinistrin的最快消除，与C57 BL/6和SV 129小鼠存在显著差异。C57 BL/6小鼠GFR随年龄增长而显著降低。在来自同一供应商的年轻和年老C57 BL/6小鼠的队列中对GFR的评价显示组间GFR值的高度一致性。我们的研究表明，所研究的技术是一种高度可重复性和可靠的方法，用于在清醒小鼠中重复测量GFR。这种温和的方法即使在老年小鼠中也很容易使用，可用于监测与年龄相关的GFR下降。
Measuring renal function in laboratory animals using blood and/or urine sampling is not only labor-intensive but puts also a strain on the animal. Several approaches for fluorescence based transcutaneous measurement of the glomerular filtration rate (GFR) in laboratory animals have been developed. They allow the measurement of GFR based on the elimination kinetics of fluorescent exogenous markers. None of the studies dealt with the reproducibility of the measurements in the same animals. Therefore, the reproducibility of a transcutaneous GFR assessment method was investigated using the fluorescent renal marker FITC-Sinistrin in conscious mice in the present study. We performed two transcutaneous GFR measurements within three days in five groups of mice (Balb/c, C57BL/6, SV129, NMRI at 3–4 months of age, and a group of 24 months old C57BL/6). Data were evaluated regarding day-to-day reproducibility as well as intra- and inter-strain variability of GFR and the impact of age on these parameters. No significant differences between the two subsequent GFR measurements were detected. Fastest elimination for FITC-Sinistrin was detected in Balb/c with significant differences to C57BL/6 and SV129 mice. GFR decreased significantly with age in C57BL/6 mice. Evaluation of GFR in cohorts of young and old C57BL/6 mice from the same supplier showed high consistency of GFR values between groups. Our study shows that the investigated technique is a highly reproducible and reliable method for repeated GFR measurements in conscious mice. This gentle method is easily used even in old mice and can be used to monitor the age-related decline in GFR.
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影响因子：
3.5
作者：
Dorshow, Richard B.;Bugaj, Joseph E.;Jones, William B.
通讯作者：
Jones, William B.
影响因子：
3.5
作者：
Poreddy, Amruta R.;Neumann, William L.;Dorshow, Richard B.
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Dorshow, Richard B.
影响因子：
19.6
作者：
Schock-Kusch, Daniel;Xie, Qing;Gretz, Norbert
通讯作者：
Gretz, Norbert
影响因子：
1.5
作者：
WILKINSON, J;FLEMING, JS;WALLER, DG
通讯作者：
WALLER, DG
影响因子：
1.5
作者：
Blake, GM;Roe, D;Lazarus, CR
通讯作者：
Lazarus, CR