Housing temperature influences the pattern of heat shock protein induction in mice following mild whole body hyperthermia.
Housing temperature influences the pattern of heat shock protein induction in mice following mild whole body hyperthermia.
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DOI：
10.3109/02656736.2014.981300
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发表时间：
2014-12
期刊：
影响因子：
--
通讯作者：
Repasky EA
中科院分区：
文献类型：
--
作者：
Eng JW;Reed CB;Kokolus KM;Repasky EA
研究小鼠对压力的反应的研究人员通常使用在国家强制执行的标准条件下饲养的小鼠作为对照。很少有研究人员关心小鼠外壳的基本物理方面是否也可能是压力源，是否能够影响实验施加的压力源的后续影响。我们最近意识到标准饲养条件可能会影响小鼠的重要生理和免疫特性。在这里，我们试图确定在标准温度（ST；22°C）与热中性温度（TT；30°C）饲养小鼠是否会影响热休克蛋白（HSP）的基线表达及其在全身加热后的典型诱导。 ST 和 TT 时 HSP 的基线表达没有显着差异。然而，在一些情况下，在加热 6 小时将核心体温升高至 39.5°C 后，我们在维持 ST 的小鼠组织中诱导 HSP70、HSP110 和 HSP90 的水平高于 ST。当给 ST 饲养的小鼠服用普萘洛尔（一种 β-肾上腺素能受体拮抗剂，临床上用于治疗高血压和应激）时，也会出现 HSP 诱导的丧失。综上所述，这些数据表明，外壳温度对全身加热后小鼠体内 HSP 的表达有显着影响，在研究小鼠应激反应时应予以考虑。
Researchers studying the response of mice to stress generally use mice housed under standard, nationally-enforced conditions as controls. Few investigators are concerned whether basic, physical aspects of mouse housing could also be a source of stress, capable of influencing the subsequent impact of an experimentally applied stressor. We have recently become aware of the potential for standard housing conditions to influence important physiological and immunological properties in mice. Here, we sought to determine whether housing mice at standard temperature (ST; 22°C) versus thermoneutral temperature (TT; 30°C) influences baseline expression of heat shock proteins (HSPs) and their typical induction following a whole body heating. There were not significant differences in baseline expression of HSPs at ST and TT. However, in several cases, we induction of HSP70, HSP110, and HSP90 in tissues of mice maintained at ST was greater than at ST, following 6 hours of heating which elevated core body temperature to 39.5°C. This loss of HSP induction was also obtained when mice housed at ST were given propranolol, a β-adrenergic receptor antagonist, used clinically to treat hypertension and stress. Taken together, these data show that housing temperature has a significant influence on the expression of HSPs in mice after whole body heating and should be considered when stress responses are studied in mice.
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影响因子：
64.8
作者：
Nguyen, Khoa D.;Qiu, Yifu;Cui, Xiaojin;Goh, Y. P. Sharon;Mwangi, Julia;David, Tovo;Mukundan, Lata;Brombacher, Frank;Locksley, Richard M.;Chawla, Ajay
通讯作者：
Chawla, Ajay
影响因子：
8
作者：
Meng, L.;Hunt, C.;Yaglom, J. A.;Gabai, V. L.;Sherman, M. Y.
通讯作者：
Sherman, M. Y.
DOI：
10.3109/02656736.2013.792126
发表时间：
2013-08
期刊：
International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group
影响因子：
--
作者：
Calderwood SK;Gong J;Stevenson MA;Murshid A
通讯作者：
Murshid A
影响因子：
6.2
作者：
Murshid A;Gong J;Stevenson MA;Calderwood SK
通讯作者：
Calderwood SK
影响因子：
15.1
作者：
Giraldo, E.;Multhoff, G.;Ortega, E.
通讯作者：
Ortega, E.