Mitochondrial dynamics and motility inside living vascular endothelial cells: role of bioenergetics.
Mitochondrial dynamics and motility inside living vascular endothelial cells: role of bioenergetics.
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DOI：
10.1007/s10439-012-0568-6
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发表时间：
2012-09
影响因子：
3.8
通讯作者：
Alevriadou, B. Rita
中科院分区：
文献类型：
--
作者：
Giedt, Randy J.;Pfeiffer, Douglas R.;Matzavinos, Anastasios;Kao, Chiu-Yen;Alevriadou, B. Rita
线粒体网络是动态的，其构象在管状连续体和片段化状态之间变化。线粒体融合/分裂之间的平衡以及细胞器运动性决定了网络形态并最终决定了线粒体/细胞功能。网络形态与不同细胞类型的能量状态有关。在这项研究中，我们研究了生物能量因素如何影响培养的血管内皮细胞（EC）的线粒体动力学/运动。EC用靶向GFP的绿色荧光蛋白（mito-GFP）转导，并暴露于氧化磷酸化（OXPHOS）或ATP合成的抑制剂。采集了延时荧光视频，并开发了计算每个时间帧每个线粒体对象的大小和速度的数学程序。我们的数据表明，线粒体内膜电位（Δ Km），糖酵解产生的ATP，以及在较小程度上，线粒体产生的ATP对于维持线粒体网络至关重要，不同的代谢应激诱导不同的形态学模式（例如，线粒体去极化是“甜甜圈”形成所必需的）。线粒体运动，其特征在于布朗扩散偶尔爆发的位移幅度，在相同的条件下，导致增加裂变抑制。因此，成像/数学分析揭示了生物能量学和线粒体网络形态之间的关系;后者可能决定EC在代谢应激下的存活。
The mitochondrial network is dynamic with conformations that vary between a tubular continuum and a fragmented state. The equilibrium between mitochondrial fusion/fission, as well as the organelle motility, determine network morphology and ultimately mitochondrial/cell function. Network morphology has been linked with the energy state in different cell types. In this study, we examined how bioenergetic factors affect mitochondrial dynamics/motility in cultured vascular endothelial cells (ECs). ECs were transduced with mitochondria-targeted green fluorescent protein (mito-GFP) and exposed to inhibitors of oxidative phosphorylation (OXPHOS) or ATP synthesis. Time-lapse fluorescence videos were acquired and a mathematical program that calculates size and speed of each mitochondrial object at each time frame was developed. Our data showed that inner mitochondrial membrane potential (ΔΨm), ATP produced by glycolysis, and, to a lesser degree, ATP produced by mitochondria are critical for maintaining the mitochondrial network, and different metabolic stresses induce distinct morphological patterns (e.g., mitochondrial depolarization is necessary for “donut” formation). Mitochondrial movement, characterized by Brownian diffusion with occasional bursts in displacement magnitude, was inhibited under the same conditions that resulted in increased fission. Hence, imaging/mathematical analysis shed light on the relationship between bioenergetics and mitochondrial network morphology; the latter may determine EC survival under metabolic stress.
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影响因子：
9.2
作者：
De Vos, KJ;Allan, VJ;Sheetz, MP
通讯作者：
Sheetz, MP
影响因子：
12.4
作者：
通讯作者：
--
DOI：
10.1073/pnas.1106862108
发表时间：
2011-09-13
影响因子：
11.1
作者：
Chang, Karen T.;Niescier, Robert F.;Min, Kyung-Tai
通讯作者：
Min, Kyung-Tai
影响因子：
2.7
作者：
Collins, Tony J.
通讯作者：
Collins, Tony J.
影响因子：
5.2
作者：
Chang CR;Blackstone C
通讯作者：
Blackstone C