The short-chain fatty acid uptake fluxes by mice on a guar gum supplemented diet associate with amelioration of major biomarkers of the metabolic syndrome.
The short-chain fatty acid uptake fluxes by mice on a guar gum supplemented diet associate with amelioration of major biomarkers of the metabolic syndrome.
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补充瓜尔胶饮食的小鼠的短链脂肪酸摄取通量与代谢综合征主要生物标志物的改善相关。
DOI：
10.1371/journal.pone.0107392
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Bakker BM
中科院分区：
文献类型：
--
作者：
den Besten G;Havinga R;Bleeker A;Rao S;Gerding A;van Eunen K;Groen AK;Reijngoud DJ;Bakker BM
膳食补充各种类型的纤维的研究已经显示出对代谢综合征症状的有益作用。短链脂肪酸（SCFAs）是肠道细菌发酵膳食纤维的主要产物，被认为在其中起着关键作用。SCFAs的浓度或其代谢是否驱动这些有益效果尚不清楚。在这项研究中，我们调查了SCFA的浓度和在体内的主机摄取流量的情况下或存在的膳食纤维瓜尔胶。给C57 Bl/6J小鼠喂食补充有0%、5%、7.5%或10%纤维瓜尔胶的高脂肪饮食。为了确定对SCFA代谢的影响，将13C标记的乙酸盐、丙酸盐或丁酸盐输注到小鼠盲肠中6小时，并测量盲肠SCFA的同位素富集。在体内生产，摄取和细菌的相互转化的乙酸，丙酸和丁酸通过结合数据从三个输液实验中的一个稳态同位素模型计算。瓜尔胶治疗以剂量依赖性方式降低代谢综合征的标志物（体重、脂肪重量、甘油三酯、葡萄糖和胰岛素水平以及HOMA-IR）。此外，肝mRNA表达的基因参与胚胎发育和脂肪酸合成的瓜尔豆胶治疗剂量依赖性下降。与对照组相比，盲肠SCFA浓度增加，但在不同的瓜尔胶剂量之间没有观察到差异。因此，盲肠SCFA浓度和代谢标志物之间没有显著相关性。相反，在体内SCFA吸收通量的主机与代谢标志物呈线性相关。我们认为，在体内SCFA流量，而不是浓度，管理从代谢综合征的膳食纤维的保护。
Studies with dietary supplementation of various types of fibers have shown beneficial effects on symptoms of the metabolic syndrome. Short-chain fatty acids (SCFAs), the main products of intestinal bacterial fermentation of dietary fiber, have been suggested to play a key role. Whether the concentration of SCFAs or their metabolism drives these beneficial effects is not yet clear. In this study we investigated the SCFA concentrations and in vivo host uptake fluxes in the absence or presence of the dietary fiber guar gum. C57Bl/6J mice were fed a high-fat diet supplemented with 0%, 5%, 7.5% or 10% of the fiber guar gum. To determine the effect on SCFA metabolism, 13C-labeled acetate, propionate or butyrate were infused into the cecum of mice for 6 h and the isotopic enrichment of cecal SCFAs was measured. The in vivo production, uptake and bacterial interconversion of acetate, propionate and butyrate were calculated by combining the data from the three infusion experiments in a single steady-state isotope model. Guar gum treatment decreased markers of the metabolic syndrome (body weight, adipose weight, triglycerides, glucose and insulin levels and HOMA-IR) in a dose-dependent manner. In addition, hepatic mRNA expression of genes involved in gluconeogenesis and fatty acid synthesis decreased dose-dependently by guar gum treatment. Cecal SCFA concentrations were increased compared to the control group, but no differences were observed between the different guar gum doses. Thus, no significant correlation was found between cecal SCFA concentrations and metabolic markers. In contrast, in vivo SCFA uptake fluxes by the host correlated linearly with metabolic markers. We argue that in vivo SCFA fluxes, and not concentrations, govern the protection from the metabolic syndrome by dietary fibers.
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DOI：
10.1007/s12349-009-0056-1
发表时间：
2010-01-01
影响因子：
1.1
作者：
Cicero, Arrigo F. G.;Derosa, Giuseppe;Gaddi, Antonio V.
通讯作者：
Gaddi, Antonio V.
肠道菌群通过短链脂肪酸受体GPR43抑制胰岛素介导的脂肪积累。
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
29
作者：
Donohoe DR;Garge N;Zhang X;Sun W;O'Connell TM;Bunger MK;Bultman SJ
通讯作者：
Bultman SJ
影响因子：
4.2
作者：
HOVERSTAD, T;MIDTVEDT, T
通讯作者：
MIDTVEDT, T
影响因子：
64.5
作者：
Muzzey D;Gómez-Uribe CA;Mettetal JT;van Oudenaarden A
通讯作者：
van Oudenaarden A