Tsetse flies (Glossina morsitans morsitans) choose birthing sites guided by substrate cues with no evidence for a role of pheromones.

Tsetse flies (Glossina morsitans morsitans) choose birthing sites guided by substrate cues with no evidence for a role of pheromones.
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DOI:
10.1098/rspb.2023.0030
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发表时间:
2023-04-26
影响因子:
4.7
通讯作者:
Prieto-Godino, Lucia L.
Prieto-Godino, Lucia L.
中科院分区:
生物学1区
文献类型:
--
作者:
Adden, Andrea K.;Haines, Lee R.;Acosta-Serrano, Alvaro;Prieto-Godino, Lucia L.

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采采蝇通过传播致命疾病非洲锥虫病,对撒哈拉以南非洲国家的公共卫生和经济发展产生重大影响。不同寻常的是,采采蝇不会产卵,而是产下一个幼虫,然后立即钻进土壤化蛹。因此,雌性选择在哪里产卵对后代的生存至关重要。以前的实验室研究表明,一种假定的幼虫信息素,正戊烷,吸引怀孕的雌性莫西蛾到合适的幼虫位置。然而,这种吸引力不能在现场实验中重现。在这里,我们通过设计自然主义的实验室实验来解决这种差异,这些实验密切模仿了野外发现的物理特征。我们发现,怀孕的g.m. morsitans既不被假定的信息素所吸引,也不被放置在土壤中的蛹所吸引,这很有趣。相比之下,雌性似乎根据环境底物线索选择幼虫的位置。我们的结论是,在自然界中可能影响幼虫位置选择的许多线索中,底物特征是一个主要的决定因素,而我们未能找到信息素作用的证据。
Tsetse flies significantly impact public health and economic development in sub-Saharan African countries by transmitting the fatal disease African trypanosomiasis. Unusually, instead of laying eggs, tsetse birth a single larva that immediately burrows into the soil to pupate. Where the female chooses to larviposit is, therefore, crucial for offspring survival. Previous laboratory studies suggested that a putative larval pheromone, n-pentadecane, attracts gravid female Glossina morsitans morsitans to appropriate larviposition sites. However, this attraction could not be reproduced in field experiments. Here, we resolve this disparity by designing naturalistic laboratory experiments that closely mimic the physical characteristics found in the wild. We show that gravid G. m. morsitans were neither attracted to the putative pheromone nor, interestingly, to pupae placed in the soil. By contrast, females appear to choose larviposition sites based on environmental substrate cues. We conclude that, among the many cues that likely contribute to larviposition choice in nature, substrate features are a main determinant, while we failed to find evidence for a role of pheromones.
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