A DTC niche plexus surrounds the germline stem cell pool in Caenorhabditis elegans.
A DTC niche plexus surrounds the germline stem cell pool in Caenorhabditis elegans.
复制标题
DOI：
10.1371/journal.pone.0088372
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kimble J
中科院分区：
文献类型：
--
作者：
Byrd DT;Knobel K;Affeldt K;Crittenden SL;Kimble J
The mesenchymal distal tip cell (DTC) provides the niche for Caenorhabditis elegans germline stem cells (GSCs). The DTC has a complex cellular architecture: its cell body caps the distal gonadal end and contacts germ cells extensively, but it also includes multiple cellular processes that extend along the germline tube and intercalate between germ cells. Here we use the lag-2 DTC promoter to drive expression of myristoylated GFP, which highlights DTC membranes and permits a more detailed view of DTC architecture. We find that short processes intercalating between germ cells contact more germ cells than seen previously. We define this region of extensive niche contact with germ cells as the DTC plexus. The extent of the DTC plexus corresponds well with the previously determined extent of the GSC pool. Moreover, expression of a differentiation marker increases as germ cells move out of the plexus. Maintenance of this DTC plexus depends on the presence of undifferentiated germ cells, suggesting that germ cell state can influence niche architecture. The roles of this DTC architecture remain an open question. One idea is that the DTC plexus delivers Notch signaling to the cluster of germ cells comprising the GSC pool; another idea is that the plexus anchors GSCs at the distal end.
登录
查看更多内容
影响因子：
11.8
作者：
Eckmann, CR;Kraemer, B;Kimble, J
通讯作者：
Kimble, J
影响因子：
3.3
作者：
Crittenden, Sarah L.;Leonhard, Kimberly A.;Kimble, Judith
通讯作者：
Kimble, Judith
影响因子：
56.9
作者：
Angelo, Giana;Van Gilst, Marc R.
通讯作者：
Van Gilst, Marc R.
影响因子：
64.5
作者：
AUSTIN, J;KIMBLE, J
通讯作者：
KIMBLE, J
影响因子：
2.7
作者：
Finger, FP;Kopish, KR;White, JG
通讯作者：
White, JG