The constitutive differential transcriptome of a brain circuit for vocal learning.
The constitutive differential transcriptome of a brain circuit for vocal learning.
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DOI：
10.1186/s12864-018-4578-0
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发表时间：
2018-04-03
期刊：
影响因子：
4.4
通讯作者：
Mello CV
中科院分区：
文献类型：
--
作者：
Lovell PV;Huizinga NA;Friedrich SR;Wirthlin M;Mello CV
模仿其他生物发声的能力，也就是所谓的发声学习能力，只有少数生物拥有，包括人类和三种鸟类。人类的发声学习能力是用来学习语音和语言的。在鸣禽中，声乐学习需要一组被称为鸣叫控制系统的特殊脑核的协调活动。最近的研究已经揭示了在这些声核中表达的一些基因，但是对这个系统的转录专门化的彻底表征仍然缺失。为了确定鸣禽品种斑胸草雀鸣叫系统主要细胞核的分子专一性，我们进行了严格而全面的微阵列分析，并通过原位杂交对380个基因进行了单独分析。我们的研究确定了3300多个基因，这些基因在成年雄性鸟类的一个或多个声核中与邻近的大脑区域相比有差异调节。生物信息学分析提供了这些基因可能参与的分子途径的见解，如细胞形态发生、细胞内在兴奋性、神经传递和神经调节、轴突引导、细胞与细胞相互作用以及细胞存活，这些都是已知的对鸣叫系统的功能特性有强烈影响的。此外，对已知参与调节神经元回路发育和生理特性的特定基因家族的深入分析，可以进一步了解歌唱系统的可能调节剂。我们的研究代表了最全面的脑回路的分子特征之一，该电路的进化促进了脊椎动物的学习行为。这些数据为歌曲控制电路功能特性的可能分子决定因素提供了新的见解。它还为药理学和遗传操作提供了令人信服的目标列表，以阐明歌唱行为和声乐学习的分子调节。本文的在线版本（10.1186/s12864-018-4578-0）包含补充材料，授权用户可使用。
The ability to imitate the vocalizations of other organisms, a trait known as vocal learning, is shared by only a few organisms, including humans, where it subserves the acquisition of speech and language, and 3 groups of birds. In songbirds, vocal learning requires the coordinated activity of a set of specialized brain nuclei referred to as the song control system. Recent efforts have revealed some of the genes that are expressed in these vocal nuclei, however a thorough characterization of the transcriptional specializations of this system is still missing. We conducted a rigorous and comprehensive analysis of microarrays, and conducted a separate analysis of 380 genes by in situ hybridizations in order to identify molecular specializations of the major nuclei of the song system of zebra finches (Taeniopygia guttata), a songbird species. Our efforts identified more than 3300 genes that are differentially regulated in one or more vocal nuclei of adult male birds compared to the adjacent brain regions. Bioinformatics analyses provided insights into the possible involvement of these genes in molecular pathways such as cellular morphogenesis, intrinsic cellular excitability, neurotransmission and neuromodulation, axonal guidance and cela-to-cell interactions, and cell survival, which are known to strongly influence the functional properties of the song system. Moreover, an in-depth analysis of specific gene families with known involvement in regulating the development and physiological properties of neuronal circuits provides further insights into possible modulators of the song system. Our study represents one of the most comprehensive molecular characterizations of a brain circuit that evolved to facilitate a learned behavior in a vertebrate. The data provide novel insights into possible molecular determinants of the functional properties of the song control circuitry. It also provides lists of compelling targets for pharmacological and genetic manipulations to elucidate the molecular regulation of song behavior and vocal learning. The online version of this article (10.1186/s12864-018-4578-0) contains supplementary material, which is available to authorized users.
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影响因子：
2.5
作者：
GULLEDGE, CC;DEVICHE, P
通讯作者：
DEVICHE, P
影响因子：
--
作者：
Carleton JB;Lovell PV;McHugh A;Marzulla T;Horback KL;Mello CV
通讯作者：
Mello CV
影响因子：
16.6
作者：
Jongbloets BC;Lemstra S;Schellino R;Broekhoven MH;Parkash J;Hellemons AJ;Mao T;Giacobini P;van Praag H;De Marchis S;Ramakers GM;Pasterkamp RJ
通讯作者：
Pasterkamp RJ
影响因子：
64.8
作者：
Brainard, MS;Doupe, AJ
通讯作者：
Doupe, AJ
影响因子：
4.3
作者：
Hilliard AT;Miller JE;Horvath S;White SA
通讯作者：
White SA