Interleukin 12 and interleukin 4 control T cell adhesion to endothelial selectins through opposite effects on alpha1, 3-fucosyltransferase VII gene expression.
Interleukin 12 and interleukin 4 control T cell adhesion to endothelial selectins through opposite effects on alpha1, 3-fucosyltransferase VII gene expression.
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DOI：
10.1084/jem.188.12.2225
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发表时间：
1998-12-21
期刊：
影响因子：
--
通讯作者：
Kansas GS
中科院分区：
文献类型：
--
作者：
Wagers AJ;Waters CM;Stoolman LM;Kansas GS
α1,3-岩藻糖基转移酶 FucT-VII 对于所有三种选择素的配体形成至关重要，其表达调节这些配体的合成。短期极化辅助 T (Th)1（而非 Th2 或初始 CD4+ T 细胞）可以定位炎症部位，但这种差异的分子基础仍不清楚。在这里，我们证明初始 CD4+ T 细胞不表达 FucT-VII 并且无法结合血管选择素。我们还表明，当 CD4+ T 细胞在 Th1 极化细胞因子白细胞介素 (IL)-12 存在的情况下被激活时，FucT-VII mRNA 的水平以及与 E-和 P-选择素的结合显着增强。相反，在 IL-4（一种 Th2 极化细胞因子）存在的情况下激活 CD4+ T 细胞会抑制 FucT-VII 的表达以及与血管选择素的结合。无论极化细胞因子是否存在，T 细胞激活都会上调 Core2 转移酶 C2GnT 的表达。这些数据表明，与 Th2 细胞或初始 CD4+ T 细胞相反，Th1 细胞识别血管选择素和归巢炎症部位的选择性能力主要由单个基因 FucT-VII 的表达控制。
The α1,3-fucosyltransferase, FucT-VII, is crucial for the formation of ligands for all three selectins, and its expression regulates the synthesis of these ligands. Short-term polarized T helper (Th)1, but not Th2 or naive CD4+ T cells, can home to sites of inflammation, but the molecular basis for this difference has remained unclear. Here we show that naive CD4+ T cells do not express FucT-VII and fail to bind vascular selectins. We also show that when CD4+ T cells are activated in the presence of the Th1 polarizing cytokine interleukin (IL)-12, levels of FucT-VII mRNA and binding to E- and P-selectin are significantly augmented. In contrast, activation of CD4+ T cells in the presence of IL-4, a Th2 polarizing cytokine, inhibited FucT-VII expression and binding to vascular selectins. T cell activation upregulated expression of the Core2 transferase, C2GnT, equivalently regardless of the presence or absence of polarizing cytokines. These data indicate that the selective ability of Th1 cells, as opposed to Th2 cells or naive CD4+ T cells, to recognize vascular selectins and home to sites of inflammation is controlled principally by the expression of a single gene, FucT-VII.
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影响因子：
64.8
作者：
Austrup, F;Vestweber, D;Hamann, A
通讯作者：
Hamann, A
影响因子：
15.3
作者：
Borges, E;Tietz, W;Vestweber, D
通讯作者：
Vestweber, D
DOI：
10.1084/jem.187.6.875
发表时间：
1998-03-16
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Sallusto F;Lenig D;Mackay CR;Lanzavecchia A
通讯作者：
Lanzavecchia A
岩藻糖基转移酶FUCT-VII调节人T细胞中的E-选择蛋白配体合成。
影响因子：
7.8
作者：
Knibbs, RN;Craig, RA;Natsuka, S;Chang, A;Cameron, M;Lowe, JB;Stoolman, LM
通讯作者：
Stoolman, LM
影响因子：
64.5
作者：
Maly, P;Thall, AD;Lowe, JB
通讯作者：
Lowe, JB