Soil commensal rhizobia promote Rhizobium etli nodulation efficiency through CinR-mediated quorum sensing

Soil commensal rhizobia promote Rhizobium etli nodulation efficiency through CinR-mediated quorum sensing
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土壤共生根瘤菌通过 CinR 介导的群体感应促进根瘤菌结瘤效率

DOI:
10.1007/s00203-018-1478-2
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发表时间:
2018-02
影响因子:
2.8
通讯作者:
Zhu Jun
Zhu Jun
中科院分区:
生物学4区
文献类型:
--
作者:
Miao Junting;Zhang Na;Liu Hejun;Wang Hui;Zhong Zengtao;Zhu Jun

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根际微生物群落是由多种直接与植物相互作用的微生物组成的。我们试图更好地了解根瘤菌在最初的共生期如何与其他土壤细菌相互作用。在这项研究中,我们调查了土壤共生物,特别是其他根瘤菌,如何影响根瘤菌和普通菜豆的相互作用。我们发现,在未经灭菌的土壤中生长的豆类比在灭菌土壤中生长的豆类形成更多的根瘤。此外,从未经灭菌的土壤中分离到一株根瘤菌,该菌株对埃特里根瘤菌的结瘤有影响。有趣的是,我们发现当与蚕豆根瘤菌共接种时,关键的群体感应调节因子CinR对于Retli结瘤效率是重要的。此外,我们还发现,蚕豆根霉产生的群体感应信号促进了CinR介导的基因在黑麦中的表达。这些数据表明,蚕豆根结线虫对蚕豆根结线虫共生的影响可能是通过多物种细菌细胞间的通讯而起作用的。
The rhizosphere microbiome is composed of diverse microorganisms directly interacting with plants and each other. We sought to achieve a better understanding of how rhizobia interact with other soil bacteria during the initial symbiosis period. In this study, we investigated how soil commensals, particularly other rhizobia, affect Rhizobium etli-Phaseolus vulgaris interactions. We found that R. etli formed significantly more nodules on beans grown in unsterilized soil than those in sterilized soil. Furthermore, a strain identified as Rhizobium fabae, isolated from unsterilized soil, was found to affect R. etli nodulation. Interestingly, we found that the key quorum sensing regulator CinR is important for R. etli nodulation efficiency when it is co-inoculated with R. fabae. Moreover, we found that quorum sensing signals produced by R. fabae promoted CinR-mediated gene expression in R. etli. These data suggest that the effects of R. fabae on R. etli symbiosis may act through multispecies bacterial cell-cell communication.
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