Trmt112 gene expression in mouse embryonic development.
Trmt112 gene expression in mouse embryonic development.
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Trmt112 基因在小鼠胚胎发育中的表达
DOI：
10.1267/ahc.11047
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发表时间：
2012-04-26
影响因子：
2.4
通讯作者：
Wu Q
中科院分区：
文献类型：
--
作者：
Gu T;He H;Zhang Y;Han Z;Hou G;Zeng T;Liu Q;Wu Q
小鼠Trmt 11 - 2是酵母Trm 11 - 2（tRNA甲基转移酶11-2）的同源基因，最初是从RIKEN克隆的，功能尚不确定。目前已知酵母TRM 112在RNA甲基化中发挥重要作用。本研究采用原位杂交和实时定量RT-PCR（QRT-PCR）技术研究了Trmt 112的表达。全胚胎原位杂交结果显示，Trmt 112在胚胎10.5天（E10.5）至E11.5天的脑和神经系统中有较高的表达水平。在发育后期E13.5和E16.5，在各种器官和组织中显著发现了丰富的表达，包括发育中的脑、神经系统、胸腺、肺、肝、肠、肾和软骨。此外，通过QRT-PCR，Trmt 112在E9.5至E18.5的整个胚胎上持续表达，并且在E12.5、E15.5和E18.5的多个器官中高度表达。这些结果表明，Trmt 112基因在小鼠胚胎发育过程中广泛表达，可能参与多种器官组织的形态发生和多种生理功能。
Mouse Trmt112, the homologous gene of yeast Trm112 (tRNA methyltransferase 11-2), was initially cloned from RIKEN with uncertain function. The yeast TRM112 is now known to play important roles in RNA methylation. Here, we studied the expression of Trmt112 by in situ hybridization and quantitative real-time RT-PCR (QRT-PCR). A higher expression level of Trmt112 was observed in the brain and nervous system by whole mount in situ hybridization from embryonic day 10.5 (E10.5) to E11.5. At later developmental stages E13.5 and E16.5, abundant expression was prominently found in various organs and tissues including developing brain, nervous system, thymus, lung, liver, intestine, kidney, and cartilage. Furthermore, Trmt112 was persistently expressed from E9.5 to E18.5 on whole embryos and highly expressed in multiple organs at E12.5, E15.5 and E18.5 by QRT-PCR. These results showed that Trmt112 gene was highly and ubiquitously expressed during mouse embryonic development, implying that it might be involved in the morphogenesis of diverse organs and tissues and numerous physiological functions.
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DOI：
10.1093/nass/nrp152
发表时间：
2009-01-01
期刊：
Nucleic acids symposium series (2004)
影响因子：
--
作者：
Okada, Kazuki;Muneyoshi, Yuki;Hori, Hiroyuki
通讯作者：
Hori, Hiroyuki
影响因子：
3.6
作者：
Studte, Patrick;Zink, Sabrina;Schaffrath, Raffael
通讯作者：
Schaffrath, Raffael
影响因子：
3.2
作者：
Moorman, AFM;Houweling, AC;Christoffels, VM
通讯作者：
Christoffels, VM
影响因子：
7.2
作者：
Hu, Zhubing;Qin, Zhixiang;Hu, Yuxin
通讯作者：
Hu, Yuxin
影响因子：
5.3
作者：
Purushothaman, SK;Bujnicki, JM;Lapeyre, B
通讯作者：
Lapeyre, B