Development of a two-circular RNA panel as potential prognostic biomarker for gastric cancer.
Development of a two-circular RNA panel as potential prognostic biomarker for gastric cancer.
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开发双环RNA组作为胃癌潜在的预后生物标志物
DOI：
10.1186/s12967-021-03075-y
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发表时间：
2021-10-02
影响因子：
7.4
通讯作者：
Li H
中科院分区：
文献类型：
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作者：
Liu J;Zhu X;Yan M;Li H
研究背景环状RNA（CircRNA）由于其高丰度和高稳定性，近年来作为疾病生物标志物的潜在应用受到越来越多的关注。本研究旨在筛选能够预测胃癌患者术后复发和生存率的circRNA。通过实时定量PCR（qRT-PCR）检测训练集（n = 136）和验证集（n = 167）中circRNA的表达水平。采用Kaplan-Meier估计、受试者工作特征（ROC）曲线和考克斯回归分析评价circRNA对胃癌患者无复发生存期（RFS）和总生存期（OS）的预后价值。CeRNA网络预测，基因本体论（GO）和京都基因和基因组百科全书（KEGG）的分析进行的circRNAs与预后significants.ResultsA共259差异表达的circRNAs在GC患者不同RFS。我们发现两个circRNA（hsa_circ_0005092和hsa_circ_0002647）在胃癌患者中高表达，随后建立了一个术后复发和预后评估的预测模型，命名为circPanel。circPanellow患者的无复发生存期（RFS）和总生存期（OS）可能较短。我们还对hsa_circ_0005092和hsa_circ_0002647进行了circRNA-miRNA-mRNA网络预测和功能分析。CEA、CA 19 -9和CA 724）。
BackgroundCircular RNAs (circRNAs) have attracted increasing attention in recent years for their potential application as disease biomarkers due to their high abundance and stability. In this study, we attempted to screen circRNAs that can be used to predict postoperative recurrence and survival in patients with gastric cancer (GC).MethodsHigh-throughput RNA sequencing was used to identify differentially expressed circRNAs in GC patients with different prognoses. The expression level of circRNAs in the training set (n = 136) and validation set (n = 167) was detected by quantitative real-time PCR (qRT-PCR). Kaplan–Meier estimator, receiver operating characteristic (ROC) curve and cox regression analysis were used to evaluate the prognostic value of circRNAs on recurrence-free survival (RFS) and overall survival (OS) in GC patients. CeRNA network prediction, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed for the circRNAs with prognostic significance.ResultsA total of 259 differentially expressed circRNAs were identified in GC patients with different RFS. We found two circRNAs (hsa_circ_0005092 and hsa_circ_0002647) that highly expressed in GC patients with good prognoses, and subsequently established a predictive model for postoperative recurrence and prognosis evaluation, named circPanel. Patients with circPanellowmight have shorter recurrence-free survival (RFS) and overall survival (OS). We also performed circRNA-miRNA-mRNA network prediction and functional analysis for hsa_circ_0005092 and hsa_circ_0002647.ConclusionsCircPanel has the potential to be a prognostic biomarker in GC patients with greater accuracy than a single circRNA and certain traditional tumor markers (e.g., CEA, CA19-9 and CA724).
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影响因子：
4.3
作者：
Matsuoka T;Yashiro M
通讯作者：
Yashiro M
影响因子：
4.6
作者：
Bachmayr-Heyda A;Reiner AT;Auer K;Sukhbaatar N;Aust S;Bachleitner-Hofmann T;Mesteri I;Grunt TW;Zeillinger R;Pils D
通讯作者：
Pils D
DOI：
10.1007/978-3-319-42118-6_6
发表时间：
2016-01-01
期刊：
METABOLISM IN CANCER
影响因子：
--
作者：
Viale, Andrea;Draetta, Giulio F.
通讯作者：
Draetta, Giulio F.
DOI：
10.1007/s10120-006-0360-0
发表时间：
2006-01-01
期刊：
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association
影响因子：
--
作者：
Whiting, John;Sano, Takeshi;Sasako, Mitsuru
通讯作者：
Sasako, Mitsuru
FBXW7 环状 RNA 在抑制神经胶质瘤肿瘤发生中的新作用。
DOI：
10.1093/jnci/djx166
发表时间：
2018-03-01
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Yang Y;Gao X;Zhang M;Yan S;Sun C;Xiao F;Huang N;Yang X;Zhao K;Zhou H;Huang S;Xie B;Zhang N
通讯作者：
Zhang N