The development of collagen-GAG scaffold-membrane composites for tendon tissue engineering.
The development of collagen-GAG scaffold-membrane composites for tendon tissue engineering.
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用于肌腱组织工程的胶原蛋白 - 胶原型支架膜复合材料的发展。
DOI：
10.1016/j.biomaterials.2011.08.035
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发表时间：
2011-12
期刊：
影响因子：
14
通讯作者：
Harley BA
中科院分区：
文献类型：
--
作者：
Caliari SR;Ramirez MA;Harley BA
目前用于肌腱缺损的组织工程方法需要改进生物材料，以平衡微观结构和力学设计标准。胶原蛋白 - 糖胺聚糖（CG）支架作为体内再生模板和体外构建体在研究细胞行为方面已取得了相当大的成功。虽然这些支架具有许多优点，但其力学性能通常比肌腱等骨科组织低几个数量级。从自然界中力学性能高效的核 - 壳复合材料（如植物茎和豪猪刺）中获得灵感，我们制造了具有高生物活性和改善的力学完整性的核 - 壳CG复合材料。这些复合材料的特点是将低密度、各向异性的CG支架核心与高密度的CG膜壳相结合。CG膜是通过蒸发工艺制造的，该工艺可以分别调节膜的厚度和弹性模量，并且发现膜在平面内是各向同性的。然后通过冷冻干燥和随后的交联将膜与各向异性的CG支架核心相结合。结果表明，增加CG膜壳的相对厚度可使复合材料的拉伸弹性模量提高多达36倍，这与层状复合材料理论的预测相符。发现CG支架 - 膜复合材料在体外能够支持肌腱细胞的活力、增殖和代谢活动，这表明它们在保持足够渗透性的同时，力学强度也得到了提高。这项工作提出了一种有效的仿生方法，用于平衡组织工程中多孔支架对强度和生物活性的要求。
Current tissue engineering approaches for tendon defects require improved biomaterials to balance microstructural and mechanical design criteria. Collagen-glycosaminoglycan (CG) scaffolds have shown considerable success as in vivo regenerative templates and in vitro constructs to study cell behavior. While these scaffolds possess many advantageous qualities, their mechanical properties are typically orders of magnitude lower than orthopedic tissues such as tendon. Taking inspiration from mechanically efficient core–shell composites in nature such as plant stems and porcupine quills, we have created core–shell CG composites that display high bioactivity and improved mechanical integrity. These composites feature integration of a low density, anisotropic CG scaffold core with a high density, CG membrane shell. CG membranes were fabricated via an evaporative process that allowed separate tuning of membrane thickness and elastic moduli and were found to be isotropic in-plane. The membranes were then integrated with an anisotropic CG scaffold core via freeze-drying and subsequent crosslinking. Increasing the relative thickness of the CG membrane shell was shown to increase composite tensile elastic modulus by as much as a factor of 36 in a manner consistent with predictions from layered composites theory. CG scaffold-membrane composites were found to support tendon cell viability, proliferation, and metabolic activity in vitro, suggesting they maintain sufficient permeability while demonstrating improved mechanical strength. This work suggests an effective, biomimetic approach for balancing strength and bioactivity requirements of porous scaffolds for tissue engineering.
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影响因子：
14
作者：
Damink, LHHO;Dijkstra, PJ;Feijen, J
通讯作者：
Feijen, J
影响因子：
--
作者：
Farrell, E;O'Brien, FJ;Campbell, VA
通讯作者：
Campbell, VA
DOI：
10.1016/b978-012370615-7/50020-2
发表时间：
2007-01-01
期刊：
PRINCIPLES OF TISSUE ENGINEERING, 3RD EDITION
影响因子：
--
作者：
Harley, Brendan A.;Yannas, Ioannis V.
通讯作者：
Yannas, Ioannis V.
影响因子：
4.1
作者：
Kuo, Catherine K.;Tuan, Rocky S.
通讯作者：
Tuan, Rocky S.
影响因子：
4.9
作者：
Harley, Brendan A.;Lynn, Andrew K.;Gibson, Lorna J.
通讯作者：
Gibson, Lorna J.