Herpes Simplex Virus Type 1 Thymidine Kinase Gene Controls Virus Pathogenesis and Latency in the Nervous System
Herpes Simplex Virus Type 1 Thymidine Kinase Gene Controls Virus Pathogenesis and Latency in the Nervous System
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单纯疱疹病毒 1 型胸苷激酶基因控制病毒发病机制和神经系统潜伏期
DOI：
10.1007/978-1-4613-3894-9_23
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
D. Gilden
中科院分区：
文献类型：
--
作者：
Y. Becker;Y. Shtram;J. Hadar;T. Ben;A. Barasofsky;Y. Asher;E. Tabor;A. Scemama;D. Gilden
采用胸苷激酶（TK）活性不同的病毒分离株，研究了单纯疱疹病毒1型（HSV-1）在不同年龄小鼠中的致病性。野生型TK+分离物接种于4 ~ 28日龄小鼠的角膜痂上，对小鼠具有致病性。具有25% TK+分离物TK活性的突变株（指定为TK1/4）对10日龄以内的小鼠具有致病性，但对老年小鼠的致病性低于TK+病毒。没有TK活性的TK病毒杀死4 - 7天大的小鼠，但10天大的小鼠存活。因此，神经系统未分化的幼鼠易受在神经元中复制的TK-病毒的感染。TK+和TK1/4毒株感染的成年小鼠神经节中可分离到潜伏病毒，TK-毒株未分离到潜伏病毒。在pBR322质粒中克隆了TK+和TK1/4病毒株的TK基因，并对这些基因的表达程度进行了研究。在TK1/4病毒株中，在BglII-BamHI 2800 bp片段上游800 bp处发现了一个包含病毒TK基因的序列，该序列影响该基因的表达。
The pathogenicity of herpes simplex virus type 1 (HSV-1) was investigated in mice of different ages, using virus isolates that differed in the level of thymidine kinase (TK) activity. The TK+ isolate from wild type virus was pathogenic in mice aged four to 28 days when inoculated onto scarified corneas. A mutant strain (designated TK1/4) that had 25% of the TK activity of the TK+ isolate was pathogenic for mice up to 10 days of age but was less pathogenic in older mice than the TK+ virus. The TK- virus with no TK activity killed mice that were four to seven days old, but mice older than 10 days survived. Infant mice with an undifferentiated nervous system are thus susceptible to the TK- virus which replicates in the neurons. Latent virus could be isolated from the ganglia of adult mice infected with the TK+ and TK1/4 virus strains but not with the TK- virus. The TK genes of the TK+ and TK1/4 virus strains were cloned in plasrnid pBR322 and the degree of expression of these genes was investigated. A sequence of 800 bp upstream to the BglII-BamHI 2800 bp fragment containing the viral TK gene was found to affect the expression of this gene in the TK1/4 virus strain.
疱疹病毒大分子合成的调节：转录起始位点和α基因的结构域。
DOI：
10.1073/pnas.77.12.7122
发表时间：
1980
影响因子：
11.1
作者：
Mackem,S;Roizman,B
通讯作者：
Roizman,B
单纯疱疹病毒胸苷激酶基因在大肠杆菌 K-12 中的功能表达。
影响因子：
3.5
作者：
Kit,S;Otsuka,H;Qavi,H;Kit,M
通讯作者：
Kit,M
1型单纯疱疹病毒胸苷激酶基因的核苷酸序列。
DOI：
10.1073/pnas.78.3.1441
发表时间：
1981
影响因子：
11.1
作者：
Wagner,MJ;Sharp,JA;Summers,WC
通讯作者：
Summers,WC