Immunocytochemical evidence for co-expression of Type III IP3 receptor with signaling components of bitter taste transduction.
Immunocytochemical evidence for co-expression of Type III IP3 receptor with signaling components of bitter taste transduction.
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DOI：
10.1186/1471-2202-2-6
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发表时间：
2001
期刊：
影响因子：
2.4
通讯作者：
Kinnamon SC
中科院分区：
文献类型：
--
作者：
Clapp TR;Stone LM;Margolskee RF;Kinnamon SC
味觉感受器细胞负责将化学刺激转换成电信号，从而产生味觉。在味觉传导中的重要第二信使是IP 3，其参与苦味和甜味传导途径。已经鉴定了苦味转导途径的几种组分，包括T2 R/TRB味觉受体、磷脂酶C β2和G蛋白亚基α-味蛋白、β3和γ13。然而，IP 3受体亚型在这一途径的身份是未知的。在本研究中，我们使用啮齿动物的味觉组织的免疫细胞化学，以确定在味觉细胞中表达的IP 3受体，并检查IP 3R 3的味蕾表达模式。针对I、II和III型IP 3受体的抗体在大鼠和小鼠轮廓乳头的切片上进行测试。在这两个物种的味觉细胞的一个大的子集中发现了III型IP 3受体（IP 3R 3）的强大的细胞质标记。与此相反，很少或没有免疫反应性被视为与抗体对I型或II型IP 3受体。为了研究IP 3R 3在苦味转导中的潜在作用，我们使用双标记免疫细胞化学来确定IP 3R 3是否在表达其他苦味信号传导组分的相同细胞亚群中表达。IP 3R 3免疫反应阳性的味细胞也对PLCβ2和γ13免疫反应。α-味蛋白免疫反应性存在于IP 3R 3、PLCβ2和γ13阳性细胞的子集中。IP 3R 3是味觉细胞中表达的IP 3受体的主要形式，我们的数据表明它在苦味转导中起着重要作用。
Taste receptor cells are responsible for transducing chemical stimuli into electrical signals that lead to the sense of taste. An important second messenger in taste transduction is IP3, which is involved in both bitter and sweet transduction pathways. Several components of the bitter transduction pathway have been identified, including the T2R/TRB taste receptors, phospholipase C β2, and the G protein subunits α-gustducin, β3, and γ13. However, the identity of the IP3 receptor subtype in this pathway is not known. In the present study we used immunocytochemistry on rodent taste tissue to identify the IP3 receptors expressed in taste cells and to examine taste bud expression patterns for IP3R3. Antibodies against Type I, II, and III IP3 receptors were tested on sections of rat and mouse circumvallate papillae. Robust cytoplasmic labeling for the Type III IP3 receptor (IP3R3) was found in a large subset of taste cells in both species. In contrast, little or no immunoreactivity was seen with antibodies against the Type I or Type II IP3 receptors. To investigate the potential role of IP3R3 in bitter taste transduction, we used double-label immunocytochemistry to determine whether IP3R3 is expressed in the same subset of cells expressing other bitter signaling components. IP3R3 immunoreactive taste cells were also immunoreactive for PLCβ2 and γ13. Alpha-gustducin immunoreactivity was present in a subset of IP3R3, PLCβ2, and γ13 positive cells. IP3R3 is the dominant form of the IP3 receptor expressed in taste cells and our data suggest it plays an important role in bitter taste transduction.
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影响因子：
4.8
作者：
Giovannucci, DR;Groblewski, GE;Yule, DI
通讯作者：
Yule, DI
影响因子：
64.8
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Matsunami, H;Montmayeur, JP;Buck, LB
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Buck, LB
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Adler, E;Hoon, MA;Zuker, CS
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Zuker, CS
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2.9
作者：
SPIELMAN, AI;HUQUE, T;BRAND, JG
通讯作者：
BRAND, JG
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1.5
作者：
OTSU, H;YAMAMOTO, A;TASHIRO, Y
通讯作者：
TASHIRO, Y