Cellulose induced protein 1 (Cip1) from Trichoderma reesei enhances the enzymatic hydrolysis of pretreated lignocellulose.
Cellulose induced protein 1 (Cip1) from Trichoderma reesei enhances the enzymatic hydrolysis of pretreated lignocellulose.
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来自里氏木霉的纤维素诱导蛋白 1 (Cip1) 增强预处理木质纤维素的酶水解
DOI：
10.1186/s12934-021-01625-z
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发表时间：
2021-07-19
影响因子：
6.4
通讯作者：
Zhao J
中科院分区：
文献类型：
--
作者：
Jia H;Sun W;Li X;Zhao J
里氏木霉是目前工业化生产纤维素酶的主要菌株。纤维素诱导蛋白1(Cip1)是里氏木霉胞外蛋白中含量最丰富的蛋白之一。有关Cip1的报道主要集中在Cip1的调控及其可能的酶活性上，而对Cip1在木质纤维素酶解过程中的作用及其可能的机制尚未见报道。本研究从里氏木霉中克隆、表达和纯化了Cip1，并研究了其对几种不同预处理的木质纤维素酶解的影响。结果表明，Cip1对木质纤维素酶解有促进作用，且促进作用明显优于牛血清白蛋白(BSA)。尤其是对于木质素含量较高的木质纤维素基质，如液态热水处理的玉米秸秆和玉米芯残渣，在添加等量蛋白质的情况下，Cip1的促进效果甚至好于商品纤维素酶。金属离子锌、铜离子对酶促作用也有一定的影响。Cip1蛋白不具有裂解酶活性，但它可以破坏纤维素酶的晶体结构，减少纤维素酶在木质素上的非生产性吸附，这部分解释了Cip1对木质纤维素酶解的促进作用。里氏木霉产生的Cip1对木质纤维素预处理后的酶解有明显的促进作用，在木质素含量较高的底物中，Cip1的促进作用甚至高于商品纤维素酶。这项研究将有助于我们更好地优化纤维素酶，提高其降解木质纤维素的能力，从而降低酶解所需的酶的成本。网上版载有补充材料，可在10.1186/s12934-021-01625-z查阅。
Trichoderma reesei is currently the main strain for the commercial production of cellulase. Cellulose induced protein 1 (Cip1) is one of the most abundant proteins in extracellular proteins of T. reesei. Reported literatures about Cip1 mainly focused on the regulation of Cip1 and its possible enzyme activities, but the effect of Cip1 on the enzymatic hydrolysis of lignocellulose and possible mechanism have not still been reported. In this study, Cip1 from T. reesei was cloned, expressed and purified, and its effects on enzymatic hydrolysis of several different pretreated lignocellulose were investigated. It was found that Cip1 could promote the enzymatic hydrolysis of pretreated lignocellulose, and the promoting effect was significantly better than that of bovine serum albumin (BSA). And especially for the lignocellulosic substrate with high lignin content such as liquid hot water pretreated corn stover and corncob residue, the promoting effect of Cip1 was even better than that of the commercial cellulase when adding equal amount protein. It was also showed that the metal ions Zn2+ and Cu2+ influenced the promoting effect on enzymatic hydrolysis. The Cip1 protein had no lyase activity, but it could destroy the crystal structure of cellulose and reduce the non-productive adsorption of cellulase on lignin, which partly interpreted the promoting effect of Cip1 on enzymatic hydrolysis of lignocellulose. The Cip1 from T. reesei could significantly promote the enzymatic hydrolysis of pretreated lignocellulose, and the promotion of Cip1 was even higher than that of commercial cellulase in the enzymatic hydrolysis of the substrates with high lignin content. This study will help us to better optimize cellulase to improve its ability to degrade lignocellulose, thereby reducing the cost of enzymes required for enzymatic hydrolysis. The online version contains supplementary material available at 10.1186/s12934-021-01625-z.
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