A cascade FRET photosensitizer that enhances photodynamic therapy for ocular melanoma
A cascade FRET photosensitizer that enhances photodynamic therapy for ocular melanoma
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级联 FRET 光敏剂可增强眼部黑色素瘤的光动力治疗
DOI：
10.1016/j.nantod.2022.101684
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发表时间：
2022-12
期刊：
影响因子：
--
通讯作者：
Kam W. Leong
中科院分区：
文献类型：
--
作者：
Jing Ruan;Fang Li;Hao Tian;Jie Yu;Hongpei Deng;Shengfang Ge;Kam W. Leong
Photodynamic therapy (PDT) relies on the generation of cytotoxic singlet oxygen (1O2) from an otherwise nontoxic photosensitizer accumulated in tumor tissue to treat cancer. However, PDT of uveal melanoma, the most common adult intraocular cancer, using potent porphyrin derivatives as photosensitizers has shown limited therapeutic efficacy due to low bioavailability and1O2yield. Here, considering the uveal melanoma microenvironment of endogenous melanin, which has a high absorption between 300 nm and 500 nm, we design a carbon dot-based photosensitizer that uses multiple different pathways to generate1O2. We conjugated gadolinium (Gd)-doped carbon dots (Gd@Cdots) with the porphyrin-derivatives chlorin e6 (Ce6) and verteporfin (Ver). The resulting nanoparticle (Gd@Cdots/Ce6/Ver) generates1O2under UV irradiation mainly in cascade FRET from Gd@Cdots to Ce6 and then to Ver; the photoactivated Ver, in turn, transfers its energy to3O2to generate1O2. In human uveal melanoma MUM-2B cells in vitro, Gd@Cdots/Ce6/Ver generated 7.1- and 2.4-fold higher production of hydroxyl radical (·OH) and superoxide anions (O2-·) following UV irradiation than Gd@Cdots, indicating greater induction of tumor cell apoptosis. In an orthotopic mouse model of uveal melanoma, PDT with Gd@Cdots/Ce6/Ver showed the same trend of superior potency in tumor growth inhibition. This photosensitizer design improves the PDT efficacy of uveal melanoma by enhancing the production of1O2and should be of broad utility to other PDT.
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影响因子：
5.5
作者：
Tada DB;Baptista MS
通讯作者：
Baptista MS
影响因子：
41.2
作者：
Tsoi, Kim M.;MacParland, Sonya A.;Ma, Xue-Zhong;Spetzler, Vinzent N.;Echeverri, Juan;Ouyang, Ben;Fadel, Saleh M.;Sykes, Edward A.;Goldaracena, Nicolas;Kaths, Johann M.;Conneely, John B.;Alman, Benjamin A.;Selzner, Markus;Ostrowski, Mario A.;Adeyi, Oyedele A.;Zilman, Anton;McGilvray, Ian D.;Chan, Warren C. W.
通讯作者：
Chan, Warren C. W.
使用掺钆碳点和碳微粒通过“开关”荧光探针对葡萄糖进行高灵敏度检测
影响因子：
2.9
作者：
Hu, Meixin;Qi, Jianrong;Shen, Guangxia
通讯作者：
Shen, Guangxia
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影响因子：
9.5
作者：
Tang, Yong'an;Hu, Jun;Yang, Xiangliang
通讯作者：
Yang, Xiangliang
影响因子：
3.7
作者：
Bhattacharyya, Santanu;Barman, Monoj Kumar;Patra, Amitava
通讯作者：
Patra, Amitava