Identification of Pathways Associated with Placental Adaptation to Maternal Nutrient Restriction in Sheep.
Identification of Pathways Associated with Placental Adaptation to Maternal Nutrient Restriction in Sheep.
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DOI：
10.3390/genes11091031
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发表时间：
2020-09-02
期刊：
影响因子：
3.5
通讯作者：
Satterfield MC
中科院分区：
文献类型：
--
作者：
Edwards AK;Dunlap KA;Spencer TE;Satterfield MC
母体营养限制损害胎盘的生长和发育，但现有的证据表明，适应机制存在于营养限制（NR）母羊的一个子集，支持胎儿正常生长，不会导致宫内生长受限（IUGR）。本研究利用Affyscore基因芯片牛和羊基因组1.0 ST阵列，以确定新的胎盘基因与差异胎儿生长率在NR母羊。通过胚胎移植产生单胎妊娠，从妊娠第35天开始，母羊接受100%国家研究理事会（NRC）（对照饲喂组; n = 7）或50% NRC（NR组; n = 24）饲料，直至第125天尸检。根据第125天的胎儿体重，将来自NR母羊的胎儿分为NR非IUGR组（n = 6）和NR IUGR组（n = 6），用于微阵列分析。在鉴定的103个差异表达基因中，与IUGR胎盘组相比，NR非IUGR胎盘组中有15个上调，88个下调。生物信息学分析显示，NR非IUGR胎盘组织中上调的基因簇与细胞膜、受体和信号传导相关。与免疫反应、营养转运和代谢相关的下调基因簇。结果表明，胎盘基因表达在妊娠后期是一个改变的胎盘免疫反应，这是与增强胎儿生长，在NR母羊的亚群的指示。
Maternal nutrient restriction impairs placental growth and development, but available evidence suggests that adaptive mechanisms exist, in a subset of nutrient restricted (NR) ewes, that support normal fetal growth and do not result in intrauterine growth restriction (IUGR). This study utilized Affymetrix GeneChip Bovine and Ovine Genome 1.0 ST Arrays to identify novel placental genes associated with differential fetal growth rates within NR ewes. Singleton pregnancies were generated by embryo transfer and, beginning on Day 35 of pregnancy, ewes received either a 100% National Research Council (NRC) (control-fed group; n = 7) or 50% NRC (NR group; n = 24) diet until necropsy on Day 125. Fetuses from NR ewes were separated into NR non-IUGR (n = 6) and NR IUGR (n = 6) groups based on Day 125 fetal weight for microarray analysis. Of the 103 differentially expressed genes identified, 15 were upregulated and 88 were downregulated in NR non-IUGR compared to IUGR placentomes. Bioinformatics analysis revealed that upregulated gene clusters in NR non-IUGR placentomes associated with cell membranes, receptors, and signaling. Downregulated gene clusters associated with immune response, nutrient transport, and metabolism. Results illustrate that placentomal gene expression in late gestation is indicative of an altered placental immune response, which is associated with enhanced fetal growth, in a subpopulation of NR ewes.
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DOI：
10.1016/s0301-2115(03)00173-8
发表时间：
2003-09-22
期刊：
EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY
影响因子：
--
作者：
Lang, U;Baker, RS;Clark, KE
通讯作者：
Clark, KE
影响因子：
4.2
作者：
Lassala, Arantzatzu;Bazer, Fuller W.;Wu, Guoyao
通讯作者：
Wu, Guoyao
影响因子：
7
作者：
Dunlap KA;Brown JD;Keith AB;Satterfield MC
通讯作者：
Satterfield MC
影响因子：
3.3
作者：
Long, N. M.;Vonnahme, K. A.;Ford, S. P.
通讯作者：
Ford, S. P.
影响因子：
3.8
作者：
Edwards, Ashley K.;McKnight, Sorin M.;Satterfield, M. Carey
通讯作者：
Satterfield, M. Carey