Adenosine receptor expression and function in rat striatal cholinergic interneurons
Adenosine receptor expression and function in rat striatal cholinergic interneurons
复制标题
大鼠纹状体胆碱能中间神经元中腺苷受体的表达和功能
DOI：
10.1038/sj.bjp.0703366
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发表时间：
2000
影响因子：
7.3
通讯作者：
P. J. Richardson
中科院分区：
文献类型：
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作者：
Z. Preston;Kevin Lee;L. Widdowson;T. Freeman;A. Dixon;P. J. Richardson
胆碱能神经元的大小，膜特性，对NK 1受体激动剂（Sar 9，Met（O2）11）P物质的敏感性和胆碱乙酰转移酶mRNA的表达在大鼠纹状体切片中被确定。A1受体mRNA在60%的神经元中检测到，A2 A受体mRNA在67%（n=15）。A1受体激动剂R-N6-（2-苯基异丙基）腺苷（R-PIA）以浓度依赖性方式使胆碱能神经元超极化，对A1受体拮抗剂8-环戊基-1，3-二丙基黄嘌呤（DPCPX，100 nM）敏感。 在双重刺激实验中，A2 A受体拮抗剂8-（3-氯苯乙烯基）咖啡因（CSC，500 nM）减少了纹状体切片中[3 H]-乙酰胆碱的释放（S2/S1 0.78±0.07 vs对照组0.95±0.05），腺苷脱氨酶也是如此（S2/S1比0.69±0.05），而A1受体拮抗剂DPCPX（100 nM）则没有影响（S2/S1 1.05±0.14）。 在存在腺苷脱氨酶的情况下，腺苷A2 A受体激动剂2-对-（（羧乙基）苯乙基氨基）-5 ′-N-乙基羧酰胺腺苷（CGS 21680，10 nM）增加释放（S2/S1比值为1.03±0.05，对照组为0.88±0.05），该作用被拮抗剂CSC阻断（500 nM，S2/S1为0.68±0.05，对照组为0.73±0.08）。 荷包牡丹碱（10 μM）、沙氯芬（1 μM）和纳洛酮（10 μM）联合灌流对CGS 21680的刺激无影响（S2/S1比值0.99±0.04）。 A1受体激动剂R-PIA（100 nM）可抑制[3 H]-乙酰胆碱的释放（S2/S1比值为0.70±0.03），该作用可被DPCPX阻断（S2/S1比值为1.06±0.07）。 它的结论是，A1和A2 A受体表达的纹状体胆碱能神经元，它们是功能活跃。
Cholinergic neurons were identified in rat striatal slices by their size, membrane properties, sensitivity to the NK1 receptor agonist (Sar9, Met(O2)11) Substance P, and expression of choline acetyltransferase mRNA. A1 receptor mRNA was detected in 60% of the neurons analysed, and A2A receptor mRNA in 67% (n=15). The A1 receptor agonist R‐N6‐(2‐phenylisopropyl)adenosine (R‐PIA) hyperpolarized cholinergic neurons in a concentration dependent manner sensitive to the A1 antagonist 8‐cyclopentyl‐1,3‐dipropylxanthine (DPCPX, 100 nM). In dual stimulus experiments, the A2A receptor antagonist 8‐(3‐chlorostyryl)caffeine (CSC, 500 nM) decreased release of [3H]‐acetylcholine from striatal slices (S2/S1 0.78±0.07 versus 0.95±0.05 in control), as did adenosine deaminase (S2/S1 ratio 0.69±0.05), whereas the A1 receptor antagonist DPCPX (100 nM) had no effect (S2/S1 1.05±0.14). In the presence of adenosine deaminase the adenosine A2A receptor agonist 2‐p‐((carboxyethyl)phenylethylamino)‐5′‐N‐ethylcarboxamidoadenosine (CGS21680, 10 nM) increased release (S2/S1 ratio 1.03±0.05 versus 0.88±0.05 in control), an effect blocked by the antagonist CSC (500 nM, S2/S1 0.68±0.05, versus 0.73±0.08 with CSC alone). The combined superfusion of bicuculline (10 μM), saclofen (1 μM) and naloxone (10 μM) had no effect on the stimulation by CGS21680 (S2/S1 ratio 0.99±0.04). The A1 receptor agonist R‐PIA (100 nM) inhibited the release of [3H]‐acetylcholine (S2/S1 ratio 0.70±0.03), an effect blocked by DPCPX (S2/S1 ratio 1.06±0.07). It is concluded that both A1 and A2A receptors are expressed on striatal cholinergic neurons where they are functionally active.
影响因子：
--
作者：
REPPERT, SM;WEAVER, DR;RIVKEES, SA
通讯作者：
RIVKEES, SA
影响因子：
5.5
作者：
JIANG, ZG;NORTH, RA
通讯作者：
NORTH, RA