A New pH-Responsive Nano Micelle for Enhancing the Effect of a Hydrophobic Bactericidal Agent on Mature Streptococcus mutans Biofilm.
A New pH-Responsive Nano Micelle for Enhancing the Effect of a Hydrophobic Bactericidal Agent on Mature Streptococcus mutans Biofilm.
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一种新型 pH 响应纳米胶束，用于增强疏水性杀菌剂对成熟变形链球菌生物膜的作用
DOI：
10.3389/fmicb.2021.761583
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发表时间：
2021
影响因子：
5.2
通讯作者：
Lo ECM
中科院分区：
文献类型：
--
作者：
Zhang M;Yu Z;Lo ECM
对生物被膜的杀菌效果是目前抗菌剂面临的主要挑战。纳米载药材料可以提高生物膜的穿透性和药物的生物利用度，在生物医学领域有着重要的应用。龋病是一种典型的生物膜相关疾病，生物膜pH的酸化与龋病的发生发展密切相关。在这项研究中，pH响应的核-壳纳米胶束（mPEG-b-PDPA）能够负载疏水抗菌剂的合成和表征，包括其在酸性生物膜内递送抗菌剂的能力。用核磁共振氢谱（1H-NMR）和凝胶渗透色谱（GPC）对该嵌段共聚物的分子结构进行了表征。通过动态光散射、透射电镜、pH滴定和药物释放检测等方法对胶束的性质进行了研究。结果表明，该亲水性胶束能够释放疏水性抗菌剂贝达喹啉。在酸性环境和成熟生物膜中。48 h内未检测到对牙周细胞的细胞毒性作用。这种pH响应型胶束能够负载疏水性抗菌剂，在预防龋齿方面具有良好的临床应用前景。
The bactericidal effect on biofilm is the main challenge currently faced by antibacterial agents. Nanoscale drug-delivery materials can enhance biofilm penetrability and drug bioavailability, and have significant applications in the biomedical field. Dental caries is a typical biofilm-related disease, and the acidification of biofilm pH is closely related to the development of dental caries. In this study, a pH-responsive core-shell nano micelle (mPEG-b-PDPA) capable of loading hydrophobic antibacterial agents was synthesized and characterized, including its ability to deliver antibacterial agents within an acidic biofilm. The molecular structure of this diblock copolymer was determined by hydrogen-1 nuclear magnetic resonance (1H-NMR) and gel permeation chromatography (GPC). The characters of the micelles were studied by dynamic light scattering (DLS), TEM, pH titration, and drug release detection. It was found that the hydrophilic micelles could deliver bedaquiline, a hydrophobic antibacterial agent on S. mutans, in acidic environments and in mature biofilm. No cytotoxic effect on the periodontal cells was detected within 48 h. This pH-responsive micelle, being able to load hydrophobic antibacterial agent, has good clinical application potential in preventing dental caries.
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影响因子：
12.8
作者：
Choi, Okkyoung;Deng, Kathy Kanjun;Hu, Zhiqiang
通讯作者：
Hu, Zhiqiang
DOI：
10.1038/nrmicro.2017.99
发表时间：
2017-12
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Koo H;Allan RN;Howlin RP;Stoodley P;Hall-Stoodley L
通讯作者：
Hall-Stoodley L
抗菌肽 GH12 通过调节牙菌斑微生物群预防龋齿
影响因子：
4.4
作者：
Jiang, Wentao;Wang, Yufei;Zhang, Linglin
通讯作者：
Zhang, Linglin
影响因子：
21.1
作者：
Gao L;Xu T;Huang G;Jiang S;Gu Y;Chen F
通讯作者：
Chen F
影响因子：
10.2
作者：
Chen X;Guo R;Wang C;Li K;Jiang X;He H;Hong W
通讯作者：
Hong W