Isolation, identification, and color characterization of cyanidin-3-glucoside and cyanidin-3-sophoroside from red raspberry
Isolation, identification, and color characterization of cyanidin-3-glucoside and cyanidin-3-sophoroside from red raspberry
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红树莓中花青素-3-葡萄糖苷和花青素-3-槐花苷的分离、鉴定及颜色表征
DOI：
10.1007/s00217-006-0550-3
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发表时间：
2008
影响因子：
3.3
通讯作者：
Wu Jihong
中科院分区：
文献类型：
--
作者：
Liao Xiaojun;Chen Fang;Zhang Yan;Hu Xiaosong;Wu Jihong
红树莓酸化甲醇提取物经AmberliteXAD-7和SephadexLH-20柱层析分离得到主要花色苷，纯度达85%以上。此外，通过HPLC-PDA和HPLC/ESI-MS分析，确定了主要花色苷为矢车菊素-3-葡萄糖苷（Cy-3-glu）和矢车菊素-3-槐糖苷（Cy-3-soph），并研究了它们的紫外-可见吸收光谱和颜色指数。Cy-3-glu和Cy-3-soph的紫外-可见吸收光谱在所有pH值下均相似，随着pH值的升高，吸收光谱逐渐红移。然而，Cy-3-soph的摩尔吸光系数低于Cy-3-glu的，随着pH的增加，除了在pH 4.6和5.7。用CIEL *a*B* 坐标、L *（亮度）、C*（色度）和H °（色相角）描述了Cy-3-glu和Cy-3-soph在缓冲溶液中的颜色指数。L * 和C * 值具有相似的变化规律。Cy-3-glu和Cy-3-soph的L * 和C * 值随pH的增加而增加，在pH 5.7时达到最大值。之后，L * 和C * 值呈现出随pH从6.5增加到7.6而减小的趋势。然而，随着pH从1.0增加到5.7，Cy-3-glu和Cy-3-soph的H°分别降低。之后，尽管pH值从5.7继续增加到7.6，但是对于Cy-3-glu和Cy-3-soph，H°保持恒定并且几乎相同。结果表明，花青素3位葡萄糖基和槐糖基的取代对花青素的光谱特性和颜色指数没有显著影响，但对摩尔吸光系数和C * 值有显著影响。
The major anthocyanins were isolated by Amberlite XAD-7 and Sephadex LH-20 column chromatography from the acidified, methanolic extracts of red raspberry, their purity was over 85%. Moreover, the major anthocyanins were identified as the cyanidin-3-glucoside (Cy-3-glu) and cyanidin-3-sophoroside (Cy-3-soph) using HPLC-PDA and HPLC/ESI-MS. Their UV–vis absorption spectra and color index were also investigated. Cy-3-glu and Cy-3-soph had the similar UV–vis absorption spectra in all investigated pH values, the bathochromic shift occurred progressively in the spectra with pH increase. However, the molar absorptivity of Cy-3-soph was lower than that of Cy-3-glu as pH increased except at pH 4.6 and 5.7. The color index of Cy-3-glu and Cy-3-soph in buffer solution were described byCIEL*a*b*coordinatesL*(lightness),C*(chroma) andH°(hue angle). TheL*andC*values had the similar change behavior. With increasing pH, theL*andC*values of Cy-3-glu and Cy-3-soph increased and reached a maximum at pH 5.7. Afterwards, theL*andC*values presented a decrease with increasing pH from 6.5 to 7.6. However,H°decreased for Cy-3-glu and Cy-3-soph with pH increasing from 1.0 to 5.7, respectively. Afterwards,H°kept constant and nearly identical for Cy-3-glu and Cy-3-soph although pH value continued increasing from 5.7 to 7.6. It could be concluded that the glucosyl and sophorosyl substitution at 3-position of cyanidin had no significant effect on spectra characteristics and color indices of anthocyanins except the molar absorptivity and theC*value.
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影响因子：
4.1
作者：
Kähkönen, MP;Heinämäki, J;Heinonen, M
通讯作者：
Heinonen, M
DOI：
10.1002/(sici)1097-0010(199811)78:3
发表时间：
1998-11
影响因子：
4.1
作者：
J. W. Arnold;S. Senter
通讯作者：
J. W. Arnold;S. Senter
DOI：
10.1007/s002170050371
发表时间：
1999-01-01
期刊：
ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY
影响因子：
--
作者：
de Ancos, B;Gonzalez, E;Cano, MP
通讯作者：
Cano, MP
DOI：
10.1016/s1570-0232(02)00055-7
发表时间：
2002-04
期刊：
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
影响因子：
--
作者：
H. Morais;C. Ramos;E. Forgács;T. Cserháti;J. Oliviera
通讯作者：
H. Morais;C. Ramos;E. Forgács;T. Cserháti;J. Oliviera
影响因子：
6.1
作者：
Giusti, MM;Rodríguez-Saona, LE;Wrolstad, RE
通讯作者：
Wrolstad, RE