Exogenous spermidine maintains the chloroplast structure of cucumber seedlings and inhibits the degradation of photosynthetic protein complexes under high-temperature stress
Exogenous spermidine maintains the chloroplast structure of cucumber seedlings and inhibits the degradation of photosynthetic protein complexes under high-temperature stress
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外源亚精胺维持黄瓜幼苗叶绿体结构并抑制高温胁迫下光合蛋白复合物的降解
DOI：
10.1007/s11738-018-2624-9
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发表时间：
2018-02
影响因子：
2.6
通讯作者：
Sun Jin
中科院分区：
文献类型：
--
作者：
Wang Liwei;Zhou Heng;Guo Shirong;An Yahong;Shu Sheng;Sun Jin
黄瓜(Cucumis sativusL.)都是耐热园艺作物，但不耐高温。高温下黄瓜的光合作用极易受到伤害。为研究高温胁迫下外源亚精胺(Spd)对黄瓜光合作用的生理机制，采用人工气候箱高温基质培养和高温胁迫(42/32℃)条件下，用1.0mmolL−1Spd处理黄瓜品种津春2号。结果表明，外源Spd减轻了热激引起的光合作用损伤，增加了叶绿素含量，维持了叶绿体结构的相对完整。Western blotting分析表明，高温胁迫下，外源Spd抑制了黄瓜叶片中光合蛋白的降解，减缓了蛋白质复合体的解离，维持了捕光复合体的稳定性。利用十二烷基硫酸钠(十二烷基硫酸钠)-尿素-PAGE和蓝色天然聚丙烯酰胺凝胶(BN-PAGE)对22个参与不同光合作用过程的差异表达类囊体膜蛋白进行了鉴定。结果表明，外源Spd调节光合体细胞膜和法氏囊体膜蛋白的表达，使其在转录和翻译水平上发生适应性变化，抑制黄瓜叶片类囊膜蛋白的降解，维持类囊体膜结构的稳定。综上所述，这些结果表明，外源Spd通过促进类囊体膜蛋白的表达和合成，减缓LHCII-Chl和类囊体膜蛋白复合体的解离，维持光合器官结构的完整性和功能稳定性，从而减轻高温诱导的光合作用伤害。
Cucumbers (Cucumis sativusL.) are thermophilic horticulture crop but do not tolerate high temperature. The photosynthesis of cucumber under high temperature is highly susceptible to damage. To study the physiological mechanism of exogenous spermidine (Spd) on cucumber photosynthesis under heat stress, the sensitive cucumber cultivar ‘Jinchun no. 2’ was grown in substrate culture at a high temperature in an artificial climate box and treated with 1.0 mmol L−1Spd under high-temperature stress (42/32 °C). The results showed that exogenous Spd alleviated the photosynthetic damage caused by heat shock, added the chlorophyll content and maintained the chloroplast structures relatively intact. Western blotting analysis showed that exogenous Spd inhibited the degradation of photosynthetic proteins, slowed the dissociation of the protein complexes, and maintained the stability of light-harvesting complexes (LHCs) in cucumber leaves under high-temperature stress. Twenty-two differentially expressed thylakoid membrane proteins involved in different photosynthetic processes were successfully authenticated by sodium dodecyl sulphate (SDS)-urea-PAGE and Blue native-polyacrylamide gel electrophoresis (BN-PAGE). The results showed that exogenous Spd regulated the expression of photosynthetic and bursal somatic membrane proteins, which resulted in adaptive changes under high-temperature stress at the transcriptional and translational levels, inhibited the degradation of thylakoid membrane proteins in cucumber leaves, and maintained the stable structure of the thylakoid membrane. Collectively, these results suggested that exogenous Spd alleviated high temperature-induced photosynthesis damage by improving the expression and synthesis of thylakoid membrane proteins, mitigating the dissociation of LHCII–Chl and thylakoid membrane protein complexes and maintaining the integrity and functional stability of the photosynthetic organ structure.
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DOI：
10.1007/0-306-48135-9_4
发表时间：
1996
期刊：
--
影响因子：
--
作者：
B. Andersson;J. Barber
通讯作者：
B. Andersson;J. Barber
影响因子：
3.7
作者：
Gu W;Xie X;Gao S;Zhou W;Pan G;Wang G
通讯作者：
Wang G
盐胁迫对黄瓜光合器官结构和功能的影响及外源腐胺的保护
影响因子：
6.4
作者：
Shu, Sheng;Guo, Shi-Rong;Yuan, Ling-Yun
通讯作者：
Yuan, Ling-Yun
影响因子：
11.4
作者：
Standfuss，R;van Scheltinga，ACT;Kühlbrandt，W
通讯作者：
Kühlbrandt，W
影响因子：
2.9
作者：
Gill, Sarvajeet Singh;Tuteja, Narendra
通讯作者：
Tuteja, Narendra