Comparison of different cells of Haematococcus pluvialis reveals an extensive acclimation mechanism during its aging process: from a perspective of photosynthesis.
Comparison of different cells of Haematococcus pluvialis reveals an extensive acclimation mechanism during its aging process: from a perspective of photosynthesis.
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DOI：
10.1371/journal.pone.0067028
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Gu W;Xie X;Gao S;Zhou W;Pan G;Wang G
生物量占优势的绿色营养细胞（GV）和虾青素占优势的橙子静息细胞（OR）都影响着工业上最终的虾青素产量。雨生红球藻的研究表明，OR细胞在细胞和亚细胞水平上与GV细胞有很大的不同。特别是，类囊体膜在OR解体和碎片。此外，OR保存了大部分光合色素，紫黄质，花药黄质和新黄质的浓度升高。值得注意的是，温和的光合作用检测OR，即使大多数的类囊体膜被拆卸，与GV相比。然而，光系统I和II（PSI和PSII）之间的能量分配模式在OR有利于PSI，这也证实了77-K荧光。由于在OR中未检测到玉米黄质，我们将驯化作用归因于虾青素，而不是叶黄素循环。此外，蛋白质组规模的比较分析的类囊体的OR和GV表明没有光合显着的变化。然而，H. pluvialis，其中GV类囊体蛋白参与生物量积累，OR类囊体蛋白参与胁迫反应。比较分析的结论可能为利用H.雨生植物。
Both biomass dominated green vegetative cells (GV) and astaxanthin-dominated orange resting cells (OR) affect the final astaxanthin yield in industry. Examination of Haematococcus pluvialis revealed that the OR cells greatly varied from the GV cells at both cellular and subcellular levels. In particular, the thylakoid membranes in the OR were disassembled and fragmented. Furthermore, the OR conserved most of the photosynthetic pigments, with elevated concentrations of violaxanthin, antheraxanthin, and neoxanthin. Notably, moderate photosynthesis was detected in OR, even though most of the thylakoid membranes were disassembled, when compared with those in the GV. However, the energy distribution pattern between photosystem I and II (PSI and PSII) in the OR favored PSI, which was also confirmed by 77-K fluorescence. As zeaxanthin was not detected in the OR, we attribute the acclimation role to astaxanthin, instead of xanthophyll cycle. Additionally, proteomic-scale comparison analysis of thylakoids of the OR and GV indicated no photosynthetically remarkable variations. However, an extensive acclimation mechanism of H. pluvialis was proposed, in which proteins in thylakoid of GV were noted to be involved in biomass accumulation and those in OR were involved in stress response. Conclusions of the comparative analysis might provide some physiological background of OR for astaxanthin production by using H. pluvialis.
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影响因子：
2.9
作者：
BLUM, H;BEIER, H;GROSS, HJ
通讯作者：
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影响因子：
11.6
作者：
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影响因子：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1016/0926-6585(66)90059-8
发表时间：
1966-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
MURATA, N;NISHIMUR.M;TAKAMIYA, A
通讯作者：
TAKAMIYA, A